Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells.
Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells.
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DOI：
10.1038/s41598-017-13522-w
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发表时间：
2017-10-11
影响因子：
4.6
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Itakura G;Ozaki M;Nagoshi N;Kawabata S;Nishiyama Y;Sugai K;Iida T;Kashiwagi R;Ookubo T;Yastake K;Matsubayashi K;Kohyama J;Iwanami A;Matsumoto M;Nakamura M;Okano H
解决来自诱导多能干细胞（iPSC）的细胞的免疫原性仍然是使用库存同种异体细胞的细胞移植策略的重要挑战。因此，我们评估了小鼠胎儿神经干/祖细胞（胎儿-NSPCs）和iPSC衍生的神经干/祖细胞（iPSC-NSPCs）在体外和体内的免疫原性。流式细胞术显示免疫表面抗原的低表达，当同基因移植到皮下组织和脊髓中时，这些细胞在所有小鼠中存活。相反，同种异体移植到皮下组织中在所有小鼠中被排斥，并且移植到完整和损伤的脊髓中的同种异体细胞在大约20%的小鼠中存活了3个月。此外，与免疫抑制剂共同治疗后，细胞存活率增加。因此，iPSC-NSPC的免疫原性和移植后免疫动力学类似于胎儿-NSPC的免疫原性和移植后免疫动力学。
Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenitor cells (iPSC-NSPCs) both in vitro and in vivo. Flow cytometry revealed the low expression of immunological surface antigens, and these cells survived in all mice when transplanted syngeneically into subcutaneous tissue and the spinal cord. In contrast, an allogeneic transplantation into subcutaneous tissue was rejected in all mice, and allogeneic cells transplanted into intact and injured spinal cords survived for 3 months in approximately 20% of mice. In addition, cell survival was increased after co-treatment with an immunosuppressive agent. Thus, the immunogenicity and post-transplantation immunological dynamics of iPSC-NSPCs resemble those of fetus-NSPCs.
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肿瘤相关巨噬细胞促进肿瘤进展的新机制：最新研究进展和肿瘤免疫治疗的潜在靶点
影响因子：
4.1
作者：
Guo Q;Jin Z;Yuan Y;Liu R;Xu T;Wei H;Xu X;He S;Chen S;Shi Z;Hou W;Hua B
通讯作者：
Hua B
影响因子：
3.7
作者：
Kobayashi Y;Okada Y;Itakura G;Iwai H;Nishimura S;Yasuda A;Nori S;Hikishima K;Konomi T;Fujiyoshi K;Tsuji O;Toyama Y;Yamanaka S;Nakamura M;Okano H
通讯作者：
Okano H
影响因子：
6.2
作者：
Boyd AS;Wood KJ
通讯作者：
Wood KJ
影响因子：
1.2
作者：
Akesson, Elisabet;Wolmer-Solberg, Nina;Odeberg, Jenny
通讯作者：
Odeberg, Jenny
影响因子：
3.7
作者：
Itakura G;Kobayashi Y;Nishimura S;Iwai H;Takano M;Iwanami A;Toyama Y;Okano H;Nakamura M
通讯作者：
Nakamura M