Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis.
Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis.
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DOI：
10.1186/s12864-016-3206-0
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发表时间：
2016-11-03
期刊：
影响因子：
4.4
通讯作者：
Ewers C
中科院分区：
文献类型：
--
作者：
Eisenberg T;Fawzy A;Nicklas W;Semmler T;Ewers C
细毛菌科(Leptotrichiaceae)是一类相当不为人所知的细菌，其中既包含粘膜上的微生物区系，也包含重要的病原体，如引起链杆菌鼠咬伤热的念珠链杆菌。到目前为止，还没有对该家族成员进行全面的基因组研究。我们的目标是分析来自20个不同成员物种的47个基因组，以阐明系统发育方面，以及基因组和鉴别特性。我们的数据为这一组以前建立的系统发育提供了新的和可靠的基础，并使我们对基因组结构和基因功能的特征有了更深入的了解。全基因组分析显示，大多数研究中的串珠葡萄球菌菌株形成了一个异质种群，没有任何明显的聚集性。对这种高致病性鼠咬伤热生物的种下变异性分析导致检测到三个具有高分辨能力的特定变量串联分析基因座。这一非常有用和经济的工具可以直接用于临床样本，而不需要费力的事先培养。通过使用新建立的MLVA数据库，我们和预期的特定病例数据现在可以很容易地进行比较，以便更好地了解这种可能报告不足的人畜共患病的流行病学。本文的在线版本(doi：10.1186/s12864-016-3206-0)包含补充材料，授权用户可以使用。
The Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties. Our data provide a novel and reliable basis of support for previously established phylogeny from this group and give a deeper insight into characteristics of genome structure and gene functions. Full genome analyses revealed that most S. moniliformis strains under study form a heterogeneous population without any significant clustering. Analysis of infra-species variability for this highly pathogenic rat bite fever organism led to the detection of three specific variable number tandem analysis loci with high discriminatory power. This highly useful and economical tool can be directly employed in clinical samples without laborious prior cultivation. Our and prospective case-specific data can now easily be compared by using a newly established MLVA database in order to gain a better insight into the epidemiology of this presumably under-reported zoonosis. The online version of this article (doi:10.1186/s12864-016-3206-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1158/1055-9965.epi-14-0262
发表时间：
2015-03
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
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Lampe JW
DOI：
10.1099/ijsem.0.000654
发表时间：
2015-12-01
影响因子：
2.8
作者：
Eisenberg, Tobias;Glaeser, Stefanie P.;Kaempfer, Peter
通讯作者：
Kaempfer, Peter
影响因子：
2.4
作者：
Boot, R;Oosterhuis, A;Thuis, HCW
通讯作者：
Thuis, HCW
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影响因子：
3.7
作者：
Dewhirst FE;Klein EA;Thompson EC;Blanton JM;Chen T;Milella L;Buckley CM;Davis IJ;Bennett ML;Marshall-Jones ZV
通讯作者：
Marshall-Jones ZV
影响因子：
16.6
作者：
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通讯作者：
Relman DA