Sensitivity Baselines, Resistance Monitoring, and Molecular Mechanisms of the Rice False Smut Pathogen Ustilaginoidea virens to Prochloraz and Azoxystrobin in Four Regions of Southern China.
Sensitivity Baselines, Resistance Monitoring, and Molecular Mechanisms of the Rice False Smut Pathogen Ustilaginoidea virens to Prochloraz and Azoxystrobin in Four Regions of Southern China.
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DOI：
10.3390/jof9080832
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发表时间：
2023-08-08
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影响因子：
--
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稻曲病是由稻曲病菌（Ustilaginoidea virens）引起的世界性水稻真菌病害之一。咪鲜胺和嘧菌酯分别属于去甲基化抑制剂和醌类外抑制剂，常用于防治该病。在这项研究中，我们分析了100 U。中国南方的云南、四川、重庆和浙江等地的稻瘟病菌对咪鲜胺和嘧菌酯的敏感性较高。咪鲜胺和嘧菌酯的EC 50范围分别为0.004 - 0.536和0.020 - 0.510 μg/mL，平均值和标准误差分别为0.062 ± 0.008和0.120 ± 0.007 μg/mL。然而，敏感性频率分布的U。对咪鲜胺和嘧菌酯的抗性表明出现了敏感性降低的亚种群。因此，将主峰处74%和68%分离株的平均EC 50值（0.031 ± 0.001和0.078 ± 0.004 μg/mL）用作U的灵敏度基线。对咪鲜胺和嘧菌酯的敏感性。不同地理种群对嘧菌酯的敏感性存在显著差异，但不同地理种群对嘧菌酯的敏感性无相关性。对咪鲜胺和嘧菌酯的抗性。在887名美国学生中，浙江分离物5-3-1对咪鲜胺表现为中度抗性，抗性因子为22.45，而5-3-1的CYP 51基因上游1986 bp和1827 bp区域未检测到核苷酸变异。CYP 51的过表达可能是其对咪鲜胺产生抗性的原因。人工接种试验表明，5-3-1对水稻具有高致病性，说明该菌对水稻的抗性已达到了一定程度。浙江省应加强对咪鲜胺的监测和管理。
Rice false smut caused by Ustilaginoidea virens is one of the most devastating fungal diseases of rice (Oryza sativa) worldwide. Prochloraz and azoxystrobin belong to the groups of demethylation inhibitors and quinone outside inhibitors, respectively, and are commonly used for controlling this disease. In this study, we analyzed the sensitivities of 100 U. virens isolates from Yunnan, Sichuan, Chongqing, and Zhejiang in Southern China to prochloraz and azoxystrobin. The ranges of EC50 for prochloraz and azoxystrobin were 0.004−0.536 and 0.020−0.510 μg/mL, with means and standard errors of 0.062 ± 0.008 and 0.120 ± 0.007 μg/mL, respectively. However, the sensitivity frequency distributions of U. virens to prochloraz and azoxystrobin indicated the emergence of subpopulations with decreased sensitivity. Therefore, the mean EC50 values of 74% and 68% of the isolates at the main peak, 0.031 ± 0.001 and 0.078 ± 0.004 μg/mL, were used as the sensitivity baselines of U. virens to prochloraz and azoxystrobin, respectively. We found significant sensitivity differences to azoxystrobin among different geographical populations and no correlation between the sensitivities of U. virens to prochloraz and azoxystrobin. Among 887 U. virens isolates, the isolate 5-3-1 from Zhejiang showed moderate resistance to prochloraz, with a resistance factor of 22.45, while no nucleotide variation in the 1986-bp upstream or 1827-bp gene regions of CYP51 from 5-3-1 was detected. Overexpression of CYP51 is probably responsible for its resistance to prochloraz. Finally, artificial inoculation showed that 5-3-1 was highly pathogenic to rice, suggesting that the resistance of U. virens to prochloraz must be monitored and managed in Zhejiang.
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影响因子：
1.4
作者：
Chen, Yu;Zhang, Yong;Gao, Tong-Chun
通讯作者：
Gao, Tong-Chun
影响因子：
1.2
作者：
Ashizawa, Taketo
通讯作者：
Ashizawa, Taketo
影响因子：
4.2
作者：
Meng J;Sun W;Mao Z;Xu D;Wang X;Lu S;Lai D;Liu Y;Zhou L;Zhang G
通讯作者：
Zhang G
DOI：
10.1002/nt.2620020106
发表时间：
1994-01-01
期刊：
Natural Toxins
影响因子：
--
作者：
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通讯作者：
Sato, Zenji
影响因子：
4.4
作者：
Hayashi, K;Schoonbeek, HJ;De Waard, MA
通讯作者：
De Waard, MA