Comprehensive Analysis of Metabolome and Transcriptome Reveals the Regulatory Network of Coconut Nutrients.
Comprehensive Analysis of Metabolome and Transcriptome Reveals the Regulatory Network of Coconut Nutrients.
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DOI：
10.3390/metabo13060683
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发表时间：
2023-05-24
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
作者：
椰子肉因其良好的风味在市场上被广泛消费。然而，椰子肉中的营养成分及其分子调控机制缺乏全面和动态的评估。在这项研究中，代谢物的积累和基因表达的三个代表性的椰子品种属于两个亚种的超高效液相色谱/串联质谱法进行了研究。共检测到6101个特征，其中52个、8个和158个分别被鉴定为氨基酸及其衍生物、多胺和脂质。代谢途径分析表明，谷胱甘肽和α-亚麻酸是主要的差异代谢产物。转录组数据显示，5个谷胱甘肽结构基因和13个多胺调节基因的表达存在显着差异，与代谢物积累的趋势一致。加权相关网络和共表达分析表明，一个新的基因WRKY 28涉及的脂质合成的调控。这些结果拓宽了我们对椰子营养代谢的理解，并为椰子营养代谢的分子基础提供了新的见解。
Coconut flesh is widely consumed in the market for its good flavor. However, a comprehensive and dynamic assessment of the nutrients in coconut flesh and their molecular regulatory mechanisms is lacking. In this study, the metabolite accumulation and gene expression of three representative coconut cultivars belonging to two subspecies were investigated using ultra performance liquid chromatography/tandem mass spectrometry. A total of 6101 features were detected, of which 52, 8, and 158 were identified as amino acids and derivatives, polyamines, and lipids, respectively. The analysis of the metabolite pathway showed that glutathione and α-linolenate were the main differential metabolites. Transcriptome data revealed significant differences in the expression of five glutathione structural genes and thirteen polyamine-regulated genes, consistent with trends in metabolite accumulation. Weighted correlation network and co-expression analyses showed that a novel gene WRKY28 was implicated in the regulation of lipid synthesis. These results broaden our understanding of coconut nutrition metabolism and provide new insights into the molecular basis of coconut nutrition metabolism.
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