Single-Cell Transcriptomics Analysis of Human Small Antral Follicles.
Single-Cell Transcriptomics Analysis of Human Small Antral Follicles.
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DOI：
10.3390/ijms222111955
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发表时间：
2021-11-04
影响因子：
5.6
通讯作者：
Chuva de Sousa Lopes SM
中科院分区：
文献类型：
--
作者：
Fan X;Moustakas I;Bialecka M;Del Valle JS;Overeem AW;Louwe LA;Pilgram GSK;van der Westerlaken LAJ;Mei H;Chuva de Sousa Lopes SM
人类卵巢卵泡发生是一个高度调控和复杂的过程。在这一动态过程中，卵泡细胞特征的表征对于理解卵泡的命运（生长、成为显性或经历闭锁）非常重要。人类卵母细胞和颗粒细胞（GCs）在早期生长和排卵卵泡中的转录特征已经被描述过；然而，小腔卵泡中卵母细胞周围有GCs的情况尚未见研究。在这里，我们生成了一个独特的单细胞转录组数据集（SmartSeq2），该数据集由从成人卵巢分离的几个个体（非显性）小腔卵泡的卵母细胞和周围的GCs组成。我们已经确定了两种主要类型的（健康）卵泡，具有明显的卵母细胞和GC特征。利用CellphoneDB算法，我们研究了每种窦型卵泡中卵母细胞和GCs之间的转化生长因子-β (tgf -β)/骨形态发生蛋白（BMP）、无翼型（MMTV）整合位点（WNT）、NOTCH和受体酪氨酸激酶（RTK）信号通路的双向配体-受体相互作用。我们的工作不仅揭示了小窦卵泡的多样性，而且有助于填补绘制人类卵泡发生和卵子发生分子景观的空白。
Human ovarian folliculogenesis is a highly regulated and complex process. Characterization of follicular cell signatures during this dynamic process is important to understand follicle fate (to grow, become dominant, or undergo atresia). The transcriptional signature of human oocytes and granulosa cells (GCs) in early-growing and ovulatory follicles have been previously described; however, that of oocytes with surrounding GCs in small antral follicles have not been studied yet. Here, we have generated a unique dataset of single-cell transcriptomics (SmartSeq2) consisting of the oocyte with surrounding GCs from several individual (non-dominant) small antral follicles isolated from adult human ovaries. We have identified two main types of (healthy) follicles, with a distinct oocyte and GC signature. Using the CellphoneDB algorithm, we then investigated the bi-directional ligand–receptor interactions regarding the transforming growth factor-β (TGFβ)/bone morphogenetic protein (BMP), wingless-type (MMTV)-integration site (WNT), NOTCH, and receptor tyrosine kinases (RTK) signaling pathways between oocyte and GCs within each antral follicle type. Our work not only revealed the diversity of small antral follicles, but also contributes to fill the gap in mapping the molecular landscape of human folliculogenesis and oogenesis.
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影响因子：
12.4
作者：
De Cian, Marie-Cecile;Gregoire, Elodie P.;Chaboissier, Marie-Christine
通讯作者：
Chaboissier, Marie-Christine
影响因子：
6.1
作者：
Assou, Said;Anahory, Tal;Hamamah, Samir
通讯作者：
Hamamah, Samir
影响因子：
8.3
作者：
ESHRE Guideline Group on Female Fertility Preservation;Anderson RA;Amant F;Braat D;D'Angelo A;Chuva de Sousa Lopes SM;Demeestere I;Dwek S;Frith L;Lambertini M;Maslin C;Moura-Ramos M;Nogueira D;Rodriguez-Wallberg K;Vermeulen N
通讯作者：
Vermeulen N
DOI：
10.1016/j.xfre.2021.03.007
发表时间：
2021-09
期刊：
F&S reports
影响因子：
--
作者：
Hanfling SN;Parikh T;Mayhew A;Robinson E;Graham J;Gomez-Lobo V;Maher JY
通讯作者：
Maher JY
影响因子：
3.7
作者：
Hatzirodos N;Irving-Rodgers HF;Hummitzsch K;Rodgers RJ
通讯作者：
Rodgers RJ