A multi-channel localized surface plasmon resonance system for absorptiometric determination of abscisic acid by using gold nanoparticles functionalized with a polyadenine-tailed aptamer
A multi-channel localized surface plasmon resonance system for absorptiometric determination of abscisic acid by using gold nanoparticles functionalized with a polyadenine-tailed aptamer
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多通道局域表面等离子体共振系统，利用聚腺嘌呤尾适配体功能化的金纳米颗粒进行吸收测定脱落酸
DOI：
10.1007/s00604-019-4003-7
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发表时间：
2019-12
影响因子：
5.7
通讯作者：
ong
中科院分区：
文献类型：
--
作者：
Wang Shun;Zhang Hao;Li Wei;Birech Zephania;Ma Liuzheng;Li Dongxian;Li Shixin;Wang Ling;Shang Junjuan;Hu Ji;ong
描述了用于吸收光度法测定脱落酸 (ABA) 的多通道局部表面等离子共振系统。该系统利用金纳米颗粒，由宽带光源、多通道对准装置和光纤光谱仪组成。该方法基于 ABA 结合适体和 ABA 之间的特异性相互作用。这会诱导金纳米颗粒 (AuNP) 的生长，该纳米颗粒被聚腺嘌呤尾适体功能化，充当光学探针。不同浓度的 ABA 会导致所生长的 AuNP 具有不同的形态。这会导致系统记录的吸收光谱发生变化。 ABA 可以通过测量生长的 AuNP 的峰值波长偏移来量化。在优化条件下，该方法在 1 nM 至 10 μM ABA 浓度范围内显示出线性关系。检测限为 0.51 nM。与基于盐诱导的 AuNP 聚集的方法相比，ABA 测定的灵敏度大大提高。这归因于多聚 A 尾适体的使用和 AuNP 的催化能力。在实际应用中，与ELISA方法相比，测定水稻鲜叶中ABA的ABA浓度，最大相对误差为8.03%。图文摘要基于多聚A尾适体-AuNPs探针生长和多通道局域表面等离子共振系统的吸光光度法测定脱落酸的示意图。
A multi-channel localized surface plasmon resonance system is described for absorptiometric determination of abscisic acid (ABA). The system is making use of gold nanoparticles and consists of a broadband light source, a multi-channel alignment device, and a fiber spectrometer. The method is based on the specific interaction between an ABA-binding aptamer and ABA. This induces the growth of gold nanoparticles (AuNPs) functionalized with a polyadenine-tailed aptamer that act as optical probes. Different concentrations of ABA give rise to varied morphologies of grown AuNPs. This causes a change of absorption spectra which is recorded by the system. ABA can be quantified by measurement of the peak wavelength shifts of grown AuNPs. Under optimized conditions, this method shows a linear relationship in the 1 nM to 10 μM ABA concentration range. The detection limit is 0.51 nM. The sensitivity of the ABA assay is strongly improved compared to the method based on salt-induced AuNP aggregation. This is attributed to the use of a poly-A-tailed aptamer and the catalytic ability of AuNPs. In the actual application, the ABA concentration of ABA in fresh leaves of rice is measured with the maximum relative error of 8.03% in comparison with the ELISA method.Graphical abstractSchematic representation of an absorptiometric approach for determination of abscisic acid based on the growth of polyA-tailed aptamer-AuNPs probes and a multi-channel localized surface plasmon resonance system.
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影响因子：
8.4
作者：
Manzano, Marisa;Vizzini, Priya;Ionescu, Rodica Elena
通讯作者：
Ionescu, Rodica Elena
光化学沉积制备高灵敏度局域表面等离子体共振光纤传感器
影响因子：
2.4
作者：
Liu Ting;Wang Wenqi;Fei Liu;Wang Shouyu
通讯作者：
Wang Shouyu
DOI：
10.1039/b9ay00048h
发表时间：
2009-11
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Yuan-Na Li;Hai-Long Wu;Jinfang Nie;Shu-Fang Li;Yong-Jie Yu;Shu-Rong Zhang;R. Yu
通讯作者：
Yuan-Na Li;Hai-Long Wu;Jinfang Nie;Shu-Fang Li;Yong-Jie Yu;Shu-Rong Zhang;R. Yu
影响因子：
12.6
作者：
J. Kim;B. Chung
通讯作者：
J. Kim;B. Chung
影响因子：
5
作者：
I. Kaplan;G. A. Hobbs;S. S. Levinson-S.
通讯作者：
I. Kaplan;G. A. Hobbs;S. S. Levinson-S.