Identification of functional elements and regulatory circuits by Drosophila modENCODE.
Identification of functional elements and regulatory circuits by Drosophila modENCODE.
复制标题
DOI：
10.1126/science.1198374
复制
发表时间：
2010-12-24
期刊：
影响因子：
--
通讯作者：
Kellis M
中科院分区：
文献类型：
--
作者：
modENCODE Consortium;Roy S;Ernst J;Kharchenko PV;Kheradpour P;Negre N;Eaton ML;Landolin JM;Bristow CA;Ma L;Lin MF;Washietl S;Arshinoff BI;Ay F;Meyer PE;Robine N;Washington NL;Di Stefano L;Berezikov E;Brown CD;Candeias R;Carlson JW;Carr A;Jungreis I;Marbach D;Sealfon R;Tolstorukov MY;Will S;Alekseyenko AA;Artieri C;Booth BW;Brooks AN;Dai Q;Davis CA;Duff MO;Feng X;Gorchakov AA;Gu T;Henikoff JG;Kapranov P;Li R;MacAlpine HK;Malone J;Minoda A;Nordman J;Okamura K;Perry M;Powell SK;Riddle NC;Sakai A;Samsonova A;Sandler JE;Schwartz YB;Sher N;Spokony R;Sturgill D;van Baren M;Wan KH;Yang L;Yu C;Feingold E;Good P;Guyer M;Lowdon R;Ahmad K;Andrews J;Berger B;Brenner SE;Brent MR;Cherbas L;Elgin SC;Gingeras TR;Grossman R;Hoskins RA;Kaufman TC;Kent W;Kuroda MI;Orr-Weaver T;Perrimon N;Pirrotta V;Posakony JW;Ren B;Russell S;Cherbas P;Graveley BR;Lewis S;Micklem G;Oliver B;Park PJ;Celniker SE;Henikoff S;Karpen GH;Lai EC;MacAlpine DM;Stein LD;White KP;Kellis M
为了深入了解基因组信息如何转化为细胞和发育程序，果蝇模式生物DNA元件百科全书（modENCODE）项目正在全面绘制发育过程中和多个细胞系中的转录本、组蛋白修饰、染色体蛋白质、转录因子、复制蛋白质和中间体以及核小体特性图谱。我们已经生成了700多个数据集，并发现了蛋白质编码、非编码、RNA调控、复制和染色质元件，使果蝇基因组的注释部分增加了两倍多。这些元件的相关活性模式揭示了一个功能性调控网络，它预测了基因的假定新功能，揭示了阶段和组织特异性调控因子，并能够进行基因表达预测。我们的研究结果为果蝇及相关物种的定向实验和计算研究提供了基础，也为朝着全面的基因组和功能注释进行系统的数据整合提供了一个模型。
To gain insight into how genomic information is translated into cellular and developmental programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) project is comprehensively mapping transcripts, histone modifications, chromosomal proteins, transcription factors, replication proteins and intermediates, and nucleosome properties across a developmental time course and in multiple cell lines. We have generated more than 700 data sets and discovered protein-coding, noncoding, RNA regulatory, replication, and chromatin elements, more than tripling the annotated portion of the Drosophila genome. Correlated activity patterns of these elements reveal a functional regulatory network, which predicts putative new functions for genes, reveals stage- and tissue-specific regulators, and enables gene-expression prediction. Our results provide a foundation for directed experimental and computational studies in Drosophila and related species and also a model for systematic data integration toward comprehensive genomic and functional annotation.
登录
查看更多内容
影响因子：
56.9
作者：
Furlong, EEM;Andersen, EC;Scott, MP
通讯作者：
Scott, MP
影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
11.4
作者：
Carr, A;Biggin, MD
通讯作者：
Biggin, MD
影响因子：
10.5
作者：
Eaton, Matthew L.;Galani, Kyriaki;MacAlpine, David M.
通讯作者：
MacAlpine, David M.
影响因子：
7
作者：
Berezikov, Eugene;Robine, Nicolas;Lai, Eric C.
通讯作者：
Lai, Eric C.