The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling.
The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling.
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胶孢炭疽病菌的效应蛋白 CgNLP1 影响入侵并破坏坏死诱导的转录因子 HbMYB8-Like 的核定位，从而抑制植物防御信号。
DOI：
10.3389/fmicb.2022.911479
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
真菌分泌大量效应子来调节宿主防御系统。了解真菌效应子调节植物防御的分子机制对于开发新的疾病控制策略具有重要意义。在这项研究中，我们鉴定了坏死和乙烯诱导蛋白 1 (Nep1) 样蛋白 (NLP) 效应基因 CgNLP1，该基因有助于分生孢子萌发、附着胞形成、侵入生长以及胶孢炭疽菌对橡胶树的毒力。本塞姆氏烟草叶子中 CgNLP1 的瞬时表达诱导植物产生乙烯。拟南芥中CgNLP1的异位表达显着增强了对灰葡萄孢和芸苔属链格孢的抗性。橡胶树的 R2R3 型转录因子 HbMYB8-like 被鉴定为 CgNLP1.HbMYB8-like 的靶标，定位于细胞核，并诱导本塞姆氏烟草细胞死亡。 CgNLP1 破坏 HbMYB8 样的核积累并抑制 HbMYB8 样诱导的细胞死亡，这是由水杨酸 (SA) 信号通路介导的。这项研究提出了一种新策略，其中 C. gloeosporioides 利用 CgNLP1 效应子来影响入侵并抑制宿主防御调节因子 HbMYB8 样以促进感染。
Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal effectors regulate plant defense is of great importance for the development of novel strategies for disease control. In this study, we identified necrosis- and ethylene-inducing protein 1 (Nep1)-like protein (NLP) effector gene, CgNLP1, which contributed to conidial germination, appressorium formation, invasive growth, and virulence of Colletotrichum gloeosporioides to the rubber tree. Transient expression of CgNLP1 in the leaves of Nicotiana benthamiana induced ethylene production in plants. Ectopic expression of CgNLP1 in Arabidopsis significantly enhanced the resistance to Botrytis cinerea and Alternaria brassicicola. An R2R3 type transcription factor HbMYB8-like of rubber tree was identified as the target of CgNLP1.HbMYB8-like, localized on the nucleus, and induced cell death in N. benthamiana. CgNLP1 disrupted nuclear accumulation of HbMYB8-like and suppressed HbMYB8-like induced cell death, which is mediated by the salicylic acid (SA) signal pathway. This study suggested a new strategy whereby C. gloeosporioides exploited the CgNLP1 effector to affect invasion and suppress a host defense regulator HbMYB8-like to facilitate infection.
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