The role of surface charge on the uptake and biocompatibility of hydroxyapatite nanoparticles with osteoblast cells.
The role of surface charge on the uptake and biocompatibility of hydroxyapatite nanoparticles with osteoblast cells.
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DOI：
10.1088/0957-4484/22/10/105708
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发表时间：
2011-03-11
期刊：
影响因子：
3.5
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Chen L;Mccrate JM;Lee JC;Li H
本研究的目的是评估具有不同表面电荷的羟基磷灰石（HAP）纳米颗粒对MC 3 T3-E1细胞系（成骨细胞）的细胞摄取行为和体外细胞活力和增殖的影响。纳米颗粒的表面电荷通过用两种羧酸：12-氨基十二烷酸（正）和十二烷二酸（负）进行表面改性而改变。未处理的HAP纳米颗粒和十二烷酸修饰的HAP纳米颗粒（中性）用作对照。X射线衍射（XRD）结果表明，3种羧酸对HAP纳米粒子的表面修饰没有改变纳米粒子的晶体结构;傅立叶变换红外光谱（FTIR）证实了羧酸在HAP纳米粒子表面的吸附和结合; Zeta电位测定证实了3种羧酸在水基溶液中成功修饰了HAP纳米粒子的表面电荷。透射电子显微镜（TEM）图像显示，大小和形状相似的带正电荷、带负电荷和未处理的HAP纳米颗粒均能穿透细胞，且带正电荷的HAP纳米颗粒比带负电荷的HAP纳米颗粒更易被细胞摄取，这可能是由于带负电荷的HAP纳米颗粒与细胞膜之间存在相互吸引或排斥作用。中性HAP纳米颗粒由于尺寸较大，无法穿透细胞膜。MTT法和LDH法结果表明，与聚苯乙烯对照组相比，三种HAP纳米颗粒（中性、阳性和未处理）处理的MC 3 T3-E1细胞的细胞活力和细胞增殖均得到了提高，其中带正电荷的HAP纳米颗粒对细胞活力和细胞增殖的改善最为明显。总之，HAP纳米颗粒的表面电荷可以被修饰以影响HAP纳米颗粒的细胞摄取，并且不同的摄取也影响细胞的行为。这些体外实验结果也为研究HAP纳米粒在基因传递和细胞内药物传递中的应用提供了有用的信息。
The objective of this study is to evaluate the effect of hydroxyapatite (HAP) nanoparticles with different surface charges on the cellular uptake behavior and in vitro cell viability and proliferation of MC3T3-E1 cell lines (osteoblast). The nanoparticles surface charge was varied by the surface modification with two carboxylic acids: 12-aminododecanoic acid (positive) and dodecanedioic acid (negative). The untreated HAP nanoparticles and dodecanoic acid modified HAP nanoparticles (neutral) were used as the control. X-ray diffraction (XRD) revealed that surface modifications by the three carboxylic acids did not change the crystal structure of HAP nanoparticles; Fourier transform infrared spectroscopy (FTIR) confirmed the adsorption and binding of the carboxylic acids on HAP nanoparticle surface; and zeta potential measurement confirmed that the chemicals successfully modified the surface charge of HAP nanoparticles in water based solution. Transmission electron microscopy (TEM) images showed that positively charged, negatively charged and untreated HAP nanoparticles, with similar size and shape, all penetrated into the cells and cells had more uptake of HAP nanoparticles with positive charge compared to those with negative charge, which might be attributed to the attractive or repulsive interaction between the negatively charged cell membrane and positively/negatively charged HAP nanoparticles. The neutral HAP nanoparticles could not penetrate cell membrane due to the larger size. MTT assay and LDH assay results indicated that as compared with the polystyrene control, greater cell viability and cell proliferation were measured on MC3T3-E1 cells treated with the three kinds of the HAP nanoparticles (neutral, positive, and untreated), among which positively charged HAP nanoparticles shows strongest improvement for cell viability and cell proliferation. In summary, the surface charge of HAP nanoparticles can be modified to influence the cellular uptake of HAP nanoparticles and the different uptake also influence the behavior of cells. These in-vitro results may also provide useful information for investigations of HAP nanoparticles applications in the gene delivery and intracellular drug delivery.
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影响因子：
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