Bacillus licheniformis-fermented products and enramycin differentially modulate microbiota and antibiotic resistome in the cecal digesta of broilers.
Bacillus licheniformis-fermented products and enramycin differentially modulate microbiota and antibiotic resistome in the cecal digesta of broilers.
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DOI：
10.1016/j.psj.2022.102010
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发表时间：
2022-09
期刊：
影响因子：
4.4
通讯作者：
Yu, Yu -Hsiang
中科院分区：
文献类型：
--
作者：
Chen, Ying-Chu;Yu, Yu -Hsiang
由于抗生素耐药性是一个全球性的健康问题，许多国家限制在动物饲料中使用抗生素来促进生长。地衣芽孢杆菌益生菌是抗生素的潜在替代品，可提高家禽的生产性能。本研究通过宏基因组测序，研究了地衣芽孢杆菌发酵产物（BLFPs）和恩拉霉素对35日龄肉鸡盲肠消化道微生物群落组成和抗生素抗性基因（ARG）分布的影响。总共 144 只一日龄雄性肉鸡（Ross 308）被随机分配到 4 个日粮处理组，如下：基础日粮（对照 [C] 组）、基础日粮加 10 mg/kg 恩拉霉素（E 组）、基础日粮加 1 g/kg BLFP（L 组）、基础日粮加 3 g/kg BLFP（H 组），每个处理有 6 个重复笼组，每笼 6 只鸟。结果表明，H组细菌的盲肠α多样性（丰富度和均匀度）高于C组。微生物群和 ARG 组成的主坐标分析表明各组盲肠样本之间存在明显差异。在盲肠食糜中，H 组中与益生菌特性相关的活性细菌（例如卷曲乳杆菌和阿克曼氏菌）的丰度高于其他组。恩拉霉素处理促进肽（bcrA）、糖肽（vanRI）和林可酰胺（lsaE）抗性基因的表达，但抑制氨基香豆素（parY）和截短侧耳素（TaeA）抗性基因的表达。 BLFP（1 和 3 g/kg）处理抑制氨基糖苷类 (ANT(6)-Ib)、链阳菌素 (vatB) 和肽 (ugd) 抗性基因的表达，但增强大环内酯 (efrA) 和氨基香豆素 (novA) 抗性基因的表达。拟杆菌属中肽抗性基因的丰度。 H组低于C组。乳杆菌属中林可酰胺抗性基因的丰度。 E组高于其他组。这些结果表明，3 g/kg BLFP 和恩拉霉素诱导的盲肠微生物群落结构的差异变化伴随着肉鸡盲肠微生物群中携带特定 ARG 的细菌宿主丰度的变化。
Since antibiotic resistance is a global health issues, the use of antibiotics in animal feed for growth promotion has been restricted in many countries. Bacillus licheniformis probiotic is a potential alternative to antibiotics for increasing poultry performance. Through metagenomic sequencing, this study investigated the effects of B. licheniformis–fermented products (BLFPs) and enramycin on the microbial community composition and antibiotic resistance gene (ARG) distribution in the cecal digesta of broilers at the age of 35 d. In total, 144 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments as follows: basal diet (control [C] group), basal diet plus 10 mg/kg enramycin (E group), basal diet plus 1 g/kg BLFPs (L group), and basal diet plus 3 g/kg BLFPs (H group), with 6 replicate cages per treatment group and 6 birds per cage. The results indicated that the cecal alpha diversity (richness and evenness) of bacterial species was higher in the H group than in the C group. Principal coordinate analysis of microbiota and the ARG composition indicated clear differences among the cecal samples of the groups. In the cecal digesta, the abundance of active bacteria associated with probiotic properties, such as Lactobacillus crispatus and Akkermansia muciniphila, was higher in the H group than in the other groups. Enramycin treatment promoted the expression of peptide (bcrA), glycopeptide (vanRI), and lincosamide (lsaE) resistance genes but inhibited the expression of aminocoumarin (parY) and pleuromutilin (TaeA) resistance genes. BLFP (1 and 3 g/kg) treatment suppressed the expression of aminoglycoside (ANT(6)-Ib), streptogramin (vatB), and peptide (ugd) resistance genes but enhanced the expression of macrolide (efrA) and aminocoumarin (novA) resistance genes. The abundance of peptide resistance genes in Bacteroides spp. was lower in the H group than in the C group. The abundance of lincosamide resistance genes in Lactobacillus spp. was higher in the E group than in the other groups. These results demonstrated that differential changes in the structure of 3 g/kg BLFPs and enramycin-induced cecal microbial communities accompany changes in the abundance of bacterial hosts carrying specific ARGs in the cecal microbiota of broilers.
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