Peace, a MYB-like transcription factor, regulates petal pigmentation in flowering peach 'Genpei' bearing variegated and fully pigmented flowers.
Peace, a MYB-like transcription factor, regulates petal pigmentation in flowering peach 'Genpei' bearing variegated and fully pigmented flowers.
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DOI：
10.1093/jxb/ert456
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发表时间：
2014-03
影响因子：
6.9
通讯作者：
Martin C
中科院分区：
文献类型：
--
作者：
Uematsu C;Katayama H;Makino I;Inagaki A;Arakawa O;Martin C
和平的表达决定了花的颜色是否着色或杂色。尽管Peace与原花色素调节剂AtMYB 123关系密切，但Peace对桃花色素苷的生物合成具有调节作用。桃树Prunus persica cv.根平在一棵树上开粉红色和杂色的花。花色素苷生物合成相关的结构基因在粉红色花瓣中强烈表达，而在杂色花瓣中表达很弱或完全不表达。从粉红色花瓣中分离的编码MYB样基因的cDNA克隆仅在粉红色花瓣中强烈表达。通过基因枪导入该基因，在杂色花瓣的白色区域产生洋红色斑点，因此该基因被命名为Peace（桃花青素颜色增强）。和平表达的差异决定了桃花的花色模式。Peace的R2 R3 DNA结合结构域与其他植物MYB调控花青素生物合成的结构域相似。三级结构的关键氨基酸和与bHLH蛋白相互作用的基序在Peace中是保守的。系统发育分析表明，Peace与AtMYB 123（TT 2）密切相关，AtMYB 123（TT 2）在拟南芥中调节原花色素的生物合成，与单子叶植物中的花色素苷调节因子密切相关，而与双子叶植物中的花色素苷调节因子关系不大。这是第一个报告，TT 2-like R2 R3 MYB已被证明调节花青素苷的生物合成。
Peace expression determined flower colouration whether pigmented or variegated. In spite of the close relationship between Peace and AtMYB123, a proanthocyanidin regulator, Peace regulates anthocyanin biosynthesis in flowering peach. Flowering peach Prunus persica cv. Genpei bears pink and variegated flowers on a single tree. The structural genes involved in anthocyanin biosynthesis were expressed strongly in pink petals but only very weakly or not at all in variegated petals. A cDNA clone encoding a MYB-like gene, isolated from pink petals was strongly expressed only in pink petals. Introduction of this gene, via biolistics gave magenta spots in the white areas of variegated petals, therefore this gene was named as Peace (peach anthocyanin colour enhancement). Differences in Peace expression determine the pattern of flower colouration in flowering peach. The R2R3 DNA-binding domain of Peace is similar to those of other plant MYBs regulating anthocyanin biosynthesis. Key amino acids for tertiary structure and the motif for interaction with bHLH proteins were conserved in Peace. Phylogenetic analysis indicates that Peace is closely related to AtMYB123 (TT2), which regulates proanthocyanidin biosynthesis in Arabidopsis, and to anthocyanin regulators in monocots rather than to regulators in dicots. This is the first report that a TT2-like R2R3 MYB has been shown to regulate anthocyanin biosynthesis.
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影响因子：
7.2
作者：
Habu, Y;Hisatomi, Y;Lida, S
通讯作者：
Lida, S
影响因子：
7.4
作者：
Deluc, L;Barrieu, F;Hamdi, S
通讯作者：
Hamdi, S
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
5.4
作者：
DOODEMAN, M;BOERSMA, EA;BIANCHI, F
通讯作者：
BIANCHI, F
影响因子：
5.4
作者：
Katayama, H;Uematsu, C
通讯作者：
Uematsu, C