New avian paramyxoviruses type I strains identified in Africa provide new outcomes for phylogeny reconstruction and genotype classification.
New avian paramyxoviruses type I strains identified in Africa provide new outcomes for phylogeny reconstruction and genotype classification.
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DOI：
10.1371/journal.pone.0076413
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Albina E
中科院分区：
文献类型：
--
作者：
de Almeida RS;Hammoumi S;Gil P;Briand FX;Molia S;Gaidet N;Cappelle J;Chevalier V;Balança G;Traoré A;Grillet C;Maminiaina OF;Guendouz S;Dakouo M;Samaké K;Bezeid Oel M;Diarra A;Chaka H;Goutard F;Thompson P;Martinez D;Jestin V;Albina E
纽卡斯尔病（ND）是世界范围内最严重的家禽致死性疾病之一。它是由具有高度基因组多样性的禽副粘病毒1引起的。在2007年启动的一项国际监测计划框架内，从非洲的家禽和野生鸟类中收集并分析了数千份样本。从健康家禽和野鸟中分离到新城疫病毒（NDV）。然而，本研究中收集的三分之二的分离株被归类为强毒株的NDV的融合蛋白的分子分析和实验在体内的挑战与两个代表性的分离株。基于F和HN基因的系统发育分析表明，从马里和埃塞俄比亚的家禽中回收的分离物形成了新的组，本文参考Diel组描述的新命名法提出了基因型XIV和亚基因型VIf。在马达加斯加，最初在其他地方报告的基因型XI的NDV毒株的传播也得到证实。对五个非洲分离株进行全基因组测序，并根据核苷酸序列进行广泛的同源性重建。组之间的进化距离和每个簇的特定氨基酸特征使我们能够改进基因型命名法。
Newcastle disease (ND) is one of the most lethal diseases of poultry worldwide. It is caused by an avian paramyxovirus 1 that has high genomic diversity. In the framework of an international surveillance program launched in 2007, several thousand samples from domestic and wild birds in Africa were collected and analyzed. ND viruses (NDV) were detected and isolated in apparently healthy fowls and wild birds. However, two thirds of the isolates collected in this study were classified as virulent strains of NDV based on the molecular analysis of the fusion protein and experimental in vivo challenges with two representative isolates. Phylogenetic analysis based on the F and HN genes showed that isolates recovered from poultry in Mali and Ethiopia form new groups, herein proposed as genotypes XIV and sub-genotype VIf with reference to the new nomenclature described by Diel’s group. In Madagascar, the circulation of NDV strains of genotype XI, originally reported elsewhere, is also confirmed. Full genome sequencing of five African isolates was generated and an extensive phylogeny reconstruction was carried out based on the nucleotide sequences. The evolutionary distances between groups and the specific amino acid signatures of each cluster allowed us to refine the genotype nomenclature.
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影响因子：
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