Maternally involved galanin neurons in the preoptic area of the rat.
Maternally involved galanin neurons in the preoptic area of the rat.
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DOI：
10.1007/s00429-016-1246-5
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发表时间：
2017-03
影响因子：
3.1
通讯作者：
Dobolyi A
中科院分区：
文献类型：
--
作者：
Cservenák M;Kis V;Keller D;Dimén D;Menyhárt L;Oláh S;Szabó ÉR;Barna J;Renner É;Usdin TB;Dobolyi A
最近对下丘脑视前区丙氨酸神经元的选择性刺激和消融确定了它们在控制母性行为中的关键作用。在这里，我们在前联合核（ACN）中发现了一组丙氨酸神经元，在内侧视前区（MPA）中发现了一组独特的丙氨酸神经元。ACN中丙氨酸神经元共表达催产素，而MPA中不表达催产素。我们通过免疫检测参与催乳素受体信号转导的磷酸化STAT5 （pSTAT5）来评估哺乳诱导的催乳素释放的影响，发现ACN中76%的泌乳素细胞对催乳素有反应，而MPA中只有12%的泌乳素细胞对泌乳素有反应。在两个视前区，含有介导哺乳对母性动机影响的神经肽TIP39 （tuberoinfundibular peptide 39）的神经末梢在丙氨酸神经元周围大量存在。在ACN和MPA中，89%和82%的丙氨酸神经元发生了密切的体细胞偶联，平均每个细胞分别为2.9和2.6个。我们用相关光镜和电镜观察了甘丙肽神经元的组织周围神经支配。基于包埋后免疫金电镜显示的TIP39末端谷氨酸含量，这种连接是兴奋的。将顺行示踪剂生物素化右旋糖酐胺注射到表达TIP39的丘脑后层板内复复体（PIL）中，表明视前TIP39纤维起源于PIL，该纤维通过哺乳被激活。因此，母鼠视前区的丙氨酸神经元受传递哺乳相关信息的兴奋性神经元通路的支配。反过来，它们可以在地形和神经化学上分为两个不同的细胞群，其中只有一个受催乳素的影响。
Recent selective stimulation and ablation of galanin neurons in the preoptic area of the hypothalamus established their critical role in control of maternal behaviors. Here, we identified a group of galanin neurons in the anterior commissural nucleus (ACN), and a distinct group in the medial preoptic area (MPA). Galanin neurons in ACN but not the MPA co-expressed oxytocin. We used immunodetection of phosphorylated STAT5 (pSTAT5), involved in prolactin receptor signal transduction, to evaluate the effects of suckling-induced prolactin release and found that 76 % of galanin cells in ACN, but only 12 % in MPA were prolactin responsive. Nerve terminals containing tuberoinfundibular peptide 39 (TIP39), a neuropeptide that mediates effects of suckling on maternal motivation, were abundant around galanin neurons in both preoptic regions. In the ACN and MPA, 89 and 82 % of galanin neurons received close somatic appositions, with an average of 2.9 and 2.6 per cell, respectively. We observed perisomatic innervation of galanin neurons using correlated light and electron microscopy. The connection was excitatory based on the glutamate content of TIP39 terminals demonstrated by post-embedding immunogold electron microscopy. Injection of the anterograde tracer biotinylated dextran amine into the TIP39-expressing posterior intralaminar complex of the thalamus (PIL) demonstrated that preoptic TIP39 fibers originate in the PIL, which is activated by suckling. Thus, galanin neurons in the preoptic area of mother rats are innervated by an excitatory neuronal pathway that conveys suckling-related information. In turn, they can be topographically and neurochemically divided into two distinct cell groups, of which only one is affected by prolactin.
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影响因子：
3.6
作者：
Brown, R. S. E.;Herbison, A. E.;Grattan, D. R.
通讯作者：
Grattan, D. R.
影响因子：
3.3
作者：
BRAMHAM, CR;TORP, R;OTTERSEN, OP
通讯作者：
OTTERSEN, OP
影响因子：
3.2
作者：
BERRYMAN, MA;RODEWALD, RD
通讯作者：
RODEWALD, RD
影响因子：
2.2
作者：
Dief, A. El-Emam;Caldwell, J. D.;Jirikowski, G. F.
通讯作者：
Jirikowski, G. F.
影响因子：
2.5
作者：
Brown, Rosemary S. E.;Kokay, Ilona C.;Grattan, David R.
通讯作者：
Grattan, David R.