Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes.
Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes.
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DOI：
10.1371/journal.pone.0040876
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ohno H
中科院分区：
文献类型：
--
作者：
Ogasawara J;Sakurai T;Kizaki T;Ishibashi Y;Izawa T;Sumitani Y;Ishida H;Radak Z;Haga S;Ohno H
在脂肪细胞中，脂肪甘油三酯脂肪酶（ATGL）催化脂肪细胞三酰甘油三酯水解的第一步，从而调节基础和激素刺激的脂肪分解。然而，关于习惯性运动诱导白色脂肪细胞通过改变转录调节因子和脂溶辅助因子对ATGL进行适应性调节的分子机制知之甚少。将雄性Wistar大鼠随机分为2组：久坐对照组（CG）和习惯性运动组（EG）。EG每周5天在跑步机上跑步，持续9周。CG没有在跑步机上跑步。在EG中，ATGL mRNA和蛋白水平升高，与CG相比，脂肪分解显著增加，同时CGI-58与ATGL蛋白的相关性显著增加。在这些条件下，观察到过氧化物酶体增殖激活受体-2 （PPARg-2）的上调。在EG中，与CG相比，罗格列酮的加入进一步显著增加了ATGL蛋白的水平。然而，在两种类型的组中，脂肪细胞中ATGL蛋白的水平通过添加胰岛素而降低，已知胰岛素可以抑制ATGL的表达。实际上，与CG相比，EG组的血浆胰岛素水平明显降低。这些数据表明，ATGL水平的升高参与了运动诱导的原发性脂肪细胞脂肪分解的增强。这种现象背后的确切机制至少在一定程度上与PPARg-2转录激活上调有关。此外，运动引起的胰岛素循环水平降低也与习惯性运动引起的原发性脂肪细胞中ATGL水平升高有关。
In adipose cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte triacylglyceride hydrolysis, thereby regulating both basal and hormone-stimulated lipolysis. However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation of ATGL in white adipocytes via alteration in transcription regulator and lipolytic cofactors. Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL, in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG. These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes.
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DOI：
10.1111/j.1463-1326.2008.00916.x
发表时间：
2009-02
期刊：
Diabetes, obesity & metabolism
影响因子：
--
作者：
Liu LF;Purushotham A;Wendel AA;Koba K;DeIuliis J;Lee K;Belury MA
通讯作者：
Belury MA
影响因子：
3.3
作者：
IZAWA, T;KOMABAYASHI, T;TSUBOI, M
通讯作者：
TSUBOI, M
影响因子：
3.8
作者：
Lira, F. S.;Rosa, J. C.;Seelaender, M.
通讯作者：
Seelaender, M.
DOI：
10.1152/ajpendo.2000.279.3.e585
发表时间：
2000-09-01
影响因子：
5.1
作者：
Enevoldsen, LH;Stallknecht, B;Galbo, H
通讯作者：
Galbo, H
DOI：
10.1016/s0006-291x(88)80502-3
发表时间：
1988-03-30
影响因子：
3.1
作者：
IZAWA, T;KOMABAYASHI, T;KOSHIMIZU, E
通讯作者：
KOSHIMIZU, E