Electrical stimulation through conductive scaffolds for cardiomyocyte tissue engineering: Systematic review and narrative synthesis.
Electrical stimulation through conductive scaffolds for cardiomyocyte tissue engineering: Systematic review and narrative synthesis.
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DOI：
10.1111/nyas.14812
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发表时间：
2022-09
影响因子：
5.2
通讯作者：
Lewis, Rebecca
中科院分区：
文献类型：
--
作者：
Scott, Louie;Elidottir, Katrin;Jeevaratnam, Kamalan;Jurewicz, Izabela;Lewis, Rebecca
电导率对于心脏组织工程具有重要意义，并且允许在模拟心脏起搏中使用电刺激。用于组织工程的生物材料的开发可以结合标准细胞培养所不常见的物理特性，并且可以促进改善心肌细胞功能。在这篇综述中，PICOT问题提出：“外部电刺激在组织工程导电支架中的应用如何影响体外细胞培养中的心肌细胞行为？”使用系统性综述和Meta-分析指南的首选报告项目以及预定的纳入和质量评价标准，评估PubMed、Web of Science和Scopus的出版物。结果显示，碳纳米管是生物材料中最常见的导电剂，啮齿动物来源的细胞类型是最常见的心肌细胞。为了评估心肌细胞，最常使用免疫荧光，利用蛋白质，如连接蛋白43、心脏α辅肌动蛋白和心肌肌钙蛋白。确定模态平均刺激方案包括在1 Hz下施加的1-3 V方形双相50 ms脉冲，接近细胞培养结束。在体外培养中加入电刺激已经证明它是心脏组织工程的有力工具，并使研究人员更接近创造最佳的人工心脏组织结构。电导率对于心脏组织工程具有重要意义，并且允许在模拟心脏起搏中使用电刺激。用于组织工程的生物材料的开发可以结合标准细胞培养所不常见的物理特性，并且可以促进改善心肌细胞功能。在这篇综述中，PICOT问题提出：“外部电刺激在组织工程导电支架中的应用如何影响体外细胞培养中的心肌细胞行为？”
Electrical conductivity is of great significance to cardiac tissue engineering and permits the use of electrical stimulation in mimicking cardiac pacing. The development of biomaterials for tissue engineering can incorporate physical properties that are uncommon to standard cell culture and can facilitate improved cardiomyocyte function. In this review, the PICOT question asks, “How has the application of external electrical stimulation in conductive scaffolds for tissue engineering affected cardiomyocyte behavior in in vitro cell culture?” The Preferred Reporting Items for Systematic Reviews and Meta‐Analysis guidelines, with predetermined inclusion and quality appraisal criteria, were used to assess publications from PubMed, Web of Science, and Scopus. Results revealed carbon nanotubes to be the most common conductive agent in biomaterials and rodent‐sourced cell types as the most common cardiomyocytes used. To assess cardiomyocytes, immunofluorescence was used most often, utilizing proteins, such as connexin 43, cardiac α‐actinin, and cardiac troponins. It was determined that the modal average stimulation protocol comprised 1–3 V square biphasic 50‐ms pulses at 1 Hz, applied toward the end of cell culture. The addition of electrical stimulation to in vitro culture has exemplified it as a powerful tool for cardiac tissue engineering and brings researchers closer to creating optimal artificial cardiac tissue constructs. Electrical conductivity is of great significance to cardiac tissue engineering and permits the use of electrical stimulation in mimicking cardiac pacing. The development of biomaterials for tissue engineering can incorporate physical properties that are uncommon to standard cell culture and can facilitate improved cardiomyocyte function. In this review, the PICOT question asks, “How has the application of external electrical stimulation in conductive scaffolds for tissue engineering affected cardiomyocyte behavior in in vitro cell culture?”
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影响因子：
10.7
作者：
Akter M;Sikder MT;Rahman MM;Ullah AKMA;Hossain KFB;Banik S;Hosokawa T;Saito T;Kurasaki M
通讯作者：
Kurasaki M
影响因子：
3.7
作者：
Chen, M;von Mikecz, A
通讯作者：
von Mikecz, A
影响因子：
14
作者：
Hsiao, Chun-Wen;Bai, Meng-Yi;Sung, Hsing-Wen
通讯作者：
Sung, Hsing-Wen
具有多功能电子器件的工程混合心脏贴片，用于在线监测和调节组织功能。
影响因子：
41.2
作者：
Feiner R;Engel L;Fleischer S;Malki M;Gal I;Shapira A;Shacham-Diamand Y;Dvir T
通讯作者：
Dvir T
影响因子：
9.7
作者：
Esmaeili H;Patino-Guerrero A;Hasany M;Ansari MO;Memic A;Dolatshahi-Pirouz A;Nikkhah M
通讯作者：
Nikkhah M