Skin CD4(+) Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites.
Skin CD4(+) Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites.
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DOI：
10.3389/fimmu.2017.01348
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发表时间：
2017
影响因子：
7.3
通讯作者：
Karasuyama H
中科院分区：
文献类型：
--
作者：
Ohta T;Yoshikawa S;Tabakawa Y;Yamaji K;Ishiwata K;Shitara H;Taya C;Oh-Hora M;Kawano Y;Miyake K;Yamanishi Y;Yonekawa H;Watanabe N;Kanuka H;Karasuyama H
蜱是一种吸血节肢动物，是莱姆病等传染病的传播媒介。在蜱虫感染后，一些动物物种可以对随后的感染产生抗性，从而降低传播的风险。据报道，在小鼠模型中，嗜碱性粒细胞在第二次而不是第一次感染时渗透到蜱虫的进食部位，并在获得性蜱虫抗性的表达中发挥关键作用。然而，嗜碱性粒细胞向第二个蜱食部位招募的机制仍然不清楚。在这里，我们研究了负责嗜碱性粒细胞募集的细胞及其产物。在T细胞缺陷小鼠中检测到少量或没有嗜碱性粒细胞浸润，CD4+而非CD8+ T细胞过继转移重建了嗜碱性粒细胞浸润。IL-3基因的表达在第二个食蜱部位高度上调，白细胞介素-3 （IL-3）充足而非IL-3缺乏的CD4+ T细胞过继性转移使嗜碱性粒细胞浸润到T细胞缺陷小鼠身上，这表明CD4+ T细胞来源的IL-3对嗜碱性粒细胞募集至关重要。值得注意的是，IL-3+常驻CD4+记忆T细胞甚至在第二次感染之前就被检测到，在先前未感染的皮肤中，远离第一次蜱虫进食部位。综上所述，皮肤CD4+记忆T细胞局部产生的IL-3似乎在嗜碱性粒细胞募集到第二个蜱食部位的过程中起着至关重要的作用。
Ticks, blood-sucking arthropods, serve as vectors for transmission of infectious diseases including Lyme borreliosis. After tick infestation, several animal species can develop resistance to subsequent infestations, reducing the risk of transmission. In a mouse model, basophils reportedly infiltrate tick-feeding sites during the second but not first infestation and play a crucial role in the expression of acquired tick resistance. However, the mechanism underlying basophil recruitment to the second tick-feeding site remains ill-defined. Here, we investigated cells and their products responsible for the basophil recruitment. Little or no basophil infiltration was detected in T-cell-deficient mice, and adoptive transfer of CD4+ but not CD8+ T cells reconstituted it. Il3 gene expression was highly upregulated at the second tick-feeding site, and adoptive transfer of interleukin-3 (IL-3)-sufficient but not IL-3-deficient CD4+ T cells conferred the basophil infiltration on T-cell-deficient mice, indicating that the CD4+ T-cell-derived IL-3 is essential for the basophil recruitment. Notably, IL-3+ resident CD4+ memory T cells were detected even before the second infestation in previously uninfested skin distant from the first tick-feeding site. Taken together, IL-3 produced locally by skin CD4+ memory T cells appears to play a crucial role in basophil recruitment to the second tick-feeding site.
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DOI：
10.4049/jimmunol.0902447
发表时间：
2010-02-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kim S;Prout M;Ramshaw H;Lopez AF;LeGros G;Min B
通讯作者：
Min B
DOI：
10.1084/jem.20142101
发表时间：
2015-08-24
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Glennie ND;Yeramilli VA;Beiting DP;Volk SW;Weaver CT;Scott P
通讯作者：
Scott P
影响因子：
--
作者：
Baaten, Bas Jg;Li, Cheng-Rui;Bradley, Linda M
通讯作者：
Bradley, Linda M
影响因子：
64.8
作者：
MOMBAERTS, P;CLARKE, AR;TONEGAWA, S
通讯作者：
TONEGAWA, S
影响因子：
16.6
作者：
Collins N;Jiang X;Zaid A;Macleod BL;Li J;Park CO;Haque A;Bedoui S;Heath WR;Mueller SN;Kupper TS;Gebhardt T;Carbone FR
通讯作者：
Carbone FR