Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process.
Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process.
复制标题
山核桃转录组分析，揭示嫁接过程中激素信号通路的动态
DOI：
10.1186/s12864-016-3182-4
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发表时间：
2016-11-17
期刊：
影响因子：
4.4
通讯作者：
Zheng B
中科院分区：
文献类型：
--
作者：
Qiu L;Jiang B;Fang J;Shen Y;Fang Z;Rm SK;Yi K;Shen C;Yan D;Zheng B
研究背景山核桃（Carya cathayensis）是我国广泛种植的木本植物，具有很高的营养和经济价值。由于其幼年期较长，嫁接是大规模种植山核桃的有用技术。为了揭示嫁接过程中的分子机制，我们对山核桃嫁接结合的转录组进行了测序。结果在我们的研究中，六个 RNA-seq 文库总共产生了 83,676,860 个干净的短读段，包括 4.19 Gb 的序列数据。鉴定出移植过程中三个时间点的大量差异表达基因（DEG）。具体而言，7天与0天（嫁接后天）比较中的777个DEG被分为11个富集基因本体（GO）类别，14天与0天比较中的262个DEG被分为15个富集GO类别。此外，这些 DEG 构建了 PPI 网络的概述。此外，还鉴定了 20 个与生长素和细胞分裂素信号通路相关的基因，其中一些基因通过 qRT-PCR 分析进行了验证。结论我们的综合分析提供了山核桃和其他木本植物嫁接过程中涉及的候选基因和激素信号通路的基本信息。
BackgroundHickory (Carya cathayensis), a woody plant with high nutritional and economic value, is widely planted in China. Due to its long juvenile phase, grafting is a useful technique for large-scale cultivation of hickory. To reveal the molecular mechanism during the graft process, we sequenced the transcriptomes of graft union in hickory.ResultsIn our study, six RNA-seq libraries yielded a total of 83,676,860 clean short reads comprising 4.19 Gb of sequence data. A large number of differentially expressed genes (DEGs) at three time points during the graft process were identified. In detail, 777 DEGs in the 7 d vs 0 d (day after grafting) comparison were classified into 11 enriched Gene Ontology (GO) categories, and 262 DEGs in the 14 d vs 0 d comparison were classified into 15 enriched GO categories. Furthermore, an overview of the PPI network was constructed by these DEGs. In addition, 20 genes related to the auxin-and cytokinin-signaling pathways were identified, and some were validated by qRT-PCR analysis.ConclusionsOur comprehensive analysis provides basic information on the candidate genes and hormone signaling pathways involved in the graft process in hickory and other woody plants.
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影响因子：
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