Over-expression of Arabidopsis AtCHR23 chromatin remodeling ATPase results in increased variability of growth and gene expression.
Over-expression of Arabidopsis AtCHR23 chromatin remodeling ATPase results in increased variability of growth and gene expression.
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DOI：
10.1186/1471-2229-14-76
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发表时间：
2014-03-25
影响因子：
5.3
通讯作者：
Mlynarova L
中科院分区：
文献类型：
--
作者：
Folta A;Severing EI;Krauskopf J;van de Geest H;Verver J;Nap JP;Mlynarova L
植物是固着生物，它们以良好的调节方式处理它们的-有时是不利的-环境。染色质重塑涉及SWI/SNF 2型ATP酶被认为是一个重要的表观遗传机制，在不同的发育程序中的基因表达的调节和整合这些程序与环境信号的反应。在这项研究中，我们报告的作用，染色质重塑拟南芥相对于变异的增长和基因表达的关系，环境条件。与野生型相比，拟南芥中AtCHR 23 ATP酶基因已经适度（2倍）过表达导致总体生长降低。详细的分析表明，在根中，生长的减少是由于细胞伸长减少。减少生长表型需要足够的光，并通过施加故意的非生物（盐，渗透）胁迫放大。相反，AtCHR 23的敲除突变不会导致这种可见的表型效应。此外，我们发现AtCHR 23的过度表达增加了遗传相同植物群体的生长变异性。这些数据表明AtCHR 23的精确和受控表达有助于生长的稳定性或稳健性。详细的RNAseq分析表明，在AtCHR 23过表达时，与环境胁迫相关的基因子集中的基因表达变异也增加。在独立qRT-PCR分析的帮助下，在个体植物中证实了基因表达的较大变化。AtCHR 23的过表达给予拟南芥一种表型，该表型与在AtCHR 12（AtCHR 23的Parkinson）的过表达后观察到的响应于非生物胁迫的生长停滞表型显著不同。这证明了拟南芥中高度相似的ATP酶的功能亚特化。AtCHR 23的过表达增加了遗传上相同的个体之间生长的变异性，其方式与与环境应激相关的不同基因子集的表达的变异性增加一致。我们认为ATCHR 23介导的染色质重塑是植物缓冲系统的一个潜在组成部分，可以保护植物免受环境诱导的表型和转录变异的影响。
Plants are sessile organisms that deal with their -sometimes adverse- environment in well-regulated ways. Chromatin remodeling involving SWI/SNF2-type ATPases is thought to be an important epigenetic mechanism for the regulation of gene expression in different developmental programs and for integrating these programs with the response to environmental signals. In this study, we report on the role of chromatin remodeling in Arabidopsis with respect to the variability of growth and gene expression in relationship to environmental conditions. Already modest (2-fold) over-expression of the AtCHR23 ATPase gene in Arabidopsis results in overall reduced growth compared to the wild-type. Detailed analyses show that in the root, the reduction of growth is due to reduced cell elongation. The reduced-growth phenotype requires sufficient light and is magnified by applying deliberate abiotic (salt, osmotic) stress. In contrast, the knockout mutation of AtCHR23 does not lead to such visible phenotypic effects. In addition, we show that over-expression of AtCHR23 increases the variability of growth in populations of genetically identical plants. These data indicate that accurate and controlled expression of AtCHR23 contributes to the stability or robustness of growth. Detailed RNAseq analyses demonstrate that upon AtCHR23 over-expression also the variation of gene expression is increased in a subset of genes that associate with environmental stress. The larger variation of gene expression is confirmed in individual plants with the help of independent qRT-PCR analysis. Over-expression of AtCHR23 gives Arabidopsis a phenotype that is markedly different from the growth arrest phenotype observed upon over-expression of AtCHR12, the paralog of AtCHR23, in response to abiotic stress. This demonstrates functional sub-specialization of highly similar ATPases in Arabidopsis. Over-expression of AtCHR23 increases the variability of growth among genetically identical individuals in a way that is consistent with increased variability of expression of a distinct subset of genes that associate with environmental stress. We propose that ATCHR23-mediated chromatin remodeling is a potential component of a buffer system in plants that protects against environmentally-induced phenotypic and transcriptional variation.
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