Psychophysical stress increases the expression of phospho-CREB, Fos protein and neurokinin-1 receptors in superficial laminae of trigeminal subnucleus caudalis in female rats.
Psychophysical stress increases the expression of phospho-CREB, Fos protein and neurokinin-1 receptors in superficial laminae of trigeminal subnucleus caudalis in female rats.
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DOI：
10.1016/j.neulet.2010.09.054
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发表时间：
2010-12-17
影响因子：
2.5
通讯作者：
Bereiter DA
中科院分区：
文献类型：
--
作者：
Duenes SL;Thompson R;Chang Z;Okamoto K;Bereiter DA
心理应激和雌激素水平是疼痛性颞下颌关节障碍（TMJD）的危险因素；然而，这种关系的神经基础尚不清楚。本研究验证了反复强迫游泳应激和雌二醇治疗改变三叉神经尾侧亚核（Vc） cAMP反应元件结合蛋白（pCREB）磷酸化的假设，Vc是颞下颌关节感觉输入的初始部位。切除卵巢的雌性大鼠给予低剂量或高剂量雌二醇，连续3天重复强迫游泳应激，第4天给予颞下颌关节内注射芥菜油或对照物。单独强迫游泳增加了Vc浅层和深层pcreb阳性神经元的数量，而不需要雌二醇治疗或TMJ刺激。强迫游泳也增加了浅层fos阳性神经元的数量和整个背Vc的神经激肽-1受体mRNA的数量，与雌二醇治疗无关。这些结果表明，仅持续的精神物理应激就足以增加pCREB和下游调节基因的表达，这些基因与尾髓背角的兴奋性增强有关，这是一个被认为对颞下颌关节痛至关重要的脑干区域。
Psychological stress and estrogen status are risk factors to develop painful temporomandibular joint disorders (TMJD); however, the neural basis for this relationship is not known. This study tested the hypothesis that repeated forced swim stress and estradiol treatment alter the phosphorylation of cAMP responsive element binding protein (pCREB) in trigeminal subnucleus caudalis (Vc), the initial site of sensory input from the TMJ. Ovariectomized female rats were given low or high dose estradiol and subjected to repeated forced swim stress for three days and on day 4 an intra-TMJ injection of mustard oil or vehicle was given. Forced swim alone increased the number of pCREB-positive neurons, independent of estradiol treatment or TMJ stimulation, in superficial and deep laminae of Vc. Forced swim also increased the number of Fos-positive neurons in superficial laminae and neurokinin-1 receptor mRNA in whole dorsal Vc, independent of estradiol treatment. These results indicated that persistent psychophysical stress alone was sufficient to increase the expression of pCREB and downstream regulated genes associated with enhanced excitability in the caudal medullary dorsal horn, a brainstem region thought to be critical for TMJD pain.
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影响因子：
3.3
作者：
Doyle, CA;Hunt, SP
通讯作者：
Hunt, SP
影响因子：
2.9
作者：
Li, JL;Ding, YQ;Mizuno, N
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作者：
Slade, G. D.;Diatchenko, L.;Maixner, W.
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影响因子：
25
作者：
Suzuki, R;Morcuende, S;Dickenson, AH
通讯作者：
Dickenson, AH
影响因子：
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作者：
Fan, Lu;Hanbury, Rose;Cohen, Rochelle S.
通讯作者：
Cohen, Rochelle S.