Effect and Correlation of Rosa roxburghii Tratt Juice Fermented by Lactobacillus paracasei SR10-1 on Oxidative Stress and Gut Microflora Dysbiosis in Streptozotocin (STZ)-Induced Type 2 Diabetes Mellitus Mice.
Effect and Correlation of Rosa roxburghii Tratt Juice Fermented by Lactobacillus paracasei SR10-1 on Oxidative Stress and Gut Microflora Dysbiosis in Streptozotocin (STZ)-Induced Type 2 Diabetes Mellitus Mice.
复制标题
DOI：
10.3390/foods12173233
复制
发表时间：
2023-08-28
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
刺梨(Rosa roxburghii Tratt，RRT)是一种优质水果，具有多种保健功能。RRT水果饮食干预已显示出预防2型糖尿病(T2 DM)的显著潜力。采用GC-MS和16S rRNA基因测序方法，研究了副乳杆菌SR10-1发酵RRT汁(FRRT)对高糖高脂饮食和链脲佐菌素(STZ)诱导的T2 DM小鼠氧化应激、短链脂肪酸(SCFAs)和肠道微生物区系的影响。结果表明，FRRT中剂量组能显著降低T2 DM小鼠的甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、尿素氮(BUN)、肌酐(Scatinine)和丙二醛(MDA)水平(p<；0.05)，显著升高高密度脂蛋白胆固醇(HDLc)、谷胱甘肽过氧化氢酶(GSH-Px)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)水平(p<；0.05)。乙酸、丙酸、丁酸和异戊酸水平显著提高，分别提高了142.28%、428.59%、1968.66%和81.04%(p<；0.05)。浮游植物的相对丰度显著增加(p<；0.05)，变形杆菌、肠杆菌科、韦氏杆菌科、弧形杆菌和克雷伯氏菌的相对丰度显著降低(p<；0.05)。相关分析表明，短杆菌与体重(p<；0.05)、超氧化物歧化酶(p<；0.01)、过氧化氢酶(p<；0.05)、总有机碳(p<；0.05)呈显著负相关。阿克曼病与体重呈显著负相关(p<；0.05)。总之，中等剂量的FRRT可能通过逆转血脂紊乱、降低氧化应激、增加SCFAs和调节肠道微生物区系组成来改善T2 DM。中剂量FRRT可作为预防T2 DM的一种新的饮食策略。
Rosa roxburghii Tratt (RRT) is a kind of excellent fruit, with many healthy functions. RRT fruit dietary interventions have demonstrated a remarkable potential to prevent type 2 diabetes mellitus (T2DM). In the present study, the effects of Lactobacillus paracasei SR10-1 fermented RRT juice (FRRT) on the oxidative stress, short-chain fatty acids (SCFAs), and gut microbiota in T2DM mice induced by high-sugar and high-fat diets and streptozotocin (STZ) were investigated using GC–MS and 16S rRNA gene sequencing. The results showed that medium-dose FRRT intervention resulted in significantly decreased levels of TG, TC, LDL-C, BUN, creatinine, and MDA (p < 0.05) and significantly increased levels of HDL-C, GSH-PX, CAT, and SOD of T2DM mice (p < 0.05). The levels of acetic acid, propionic acid, butyric acid, and isovaleric acid were significantly increased, by 142.28%, 428.59%, 1968.66%, and 81.04% (p < 0.05), respectively. The relative abundance of Firmicutes, Lachnospiraceae, Verrucomicrobiaceae, Akkermansia, and Allobaculum was significantly increased (p < 0.05), and the relative abundance of Proteobacteria, Enterobacteriaceae, Veillonellaceae, Phascolarctobacterium, and Klebsiella was significantly decreased (p < 0.05). Correlation analysis showed that Phascolarctobacterium was significantly negatively correlated with weight (p < 0.05), SOD (p < 0.01), CAT (p < 0.05), and T-AOC (p < 0.05). Akkermansia was significantly negatively correlated with weight (p < 0.05). Conclusively, medium-dose FRRT potentially improved T2DM by reversing dyslipidemia, decreasing oxidative stress, increasing SCFAs, and regulating gut microbiota composition. The medium-dose FRRT may serve as a novel T2DM dietary strategy to prevent T2DM.
登录
查看更多内容
DOI：
10.1016/s0140-6736(14)60613-9
发表时间：
2014-06-07
期刊：
Lancet (London, England)
影响因子：
--
作者：
Ley SH;Hamdy O;Mohan V;Hu FB
通讯作者：
Hu FB
DOI：
10.3390/foods11050636
发表时间：
2022-02-22
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Chen C;Tan S;Ren T;Wang H;Dai X;Wang H
通讯作者：
Wang H
影响因子：
2.4
作者：
Aronson D;Edelman ER
通讯作者：
Edelman ER
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
8.2
作者：
Allin KH;Tremaroli V;Caesar R;Jensen BAH;Damgaard MTF;Bahl MI;Licht TR;Hansen TH;Nielsen T;Dantoft TM;Linneberg A;Jørgensen T;Vestergaard H;Kristiansen K;Franks PW;IMI-DIRECT consortium;Hansen T;Bäckhed F;Pedersen O
通讯作者：
Pedersen O