Visualization of sialidase activity in Mammalian tissues and cancer detection with a novel fluorescent sialidase substrate.
Visualization of sialidase activity in Mammalian tissues and cancer detection with a novel fluorescent sialidase substrate.
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DOI：
10.1371/journal.pone.0081941
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Minami A;Otsubo T;Ieno D;Ikeda K;Kanazawa H;Shimizu K;Ohata K;Yokochi T;Horii Y;Fukumoto H;Taguchi R;Takahashi T;Oku N;Suzuki T
唾液酸酶从唾液酸偶联物中去除唾液酸，在许多生理和病理过程中发挥重要作用。多种人类癌症都表达异常高水平的质膜相关唾液酸酶亚型，观察活的哺乳动物组织中唾液酸酶活性不仅有助于了解唾液酸酶的功能，而且有助于癌症的诊断。然而，由于哺乳动物唾液酸酶的活性明显弱于细菌和病毒唾液酸酶，因此很难检测到哺乳动物组织中的唾液酸酶活性。我们合成了一种新型的基于苯并噻唑基苯酚的唾液酸衍生物(BTP-Neu5Ac)作为唾液酸酶的荧光底物。BTP-Neu5Ac能灵敏、选择性地显示急性大鼠脑片唾液酸酶活性。BTP-Neu5Ac也能清晰地检测到小鼠结肠癌和人类结肠癌(T3-T4期)原位移植的癌细胞。结果表明，BTP-Neu5Ac可用于唾液酸酶活性的组织化学成像。
Sialidase removes sialic acid from sialoglycoconjugates and plays crucial roles in many physiological and pathological processes. Various human cancers express an abnormally high level of the plasma membrane-associated sialidase isoform.Visualization of sialidase activity in living mammalian tissues would be useful not only for understanding sialidase functions but also for cancer diagnosis. However, since enzyme activity of mammalian sialidase is remarkably weak compared with that of bacterial and viral sialidases, it has been difficult to detect sialidase activity in mammalian tissues. We synthesized a novel benzothiazolylphenol-based sialic acid derivative (BTP-Neu5Ac) as a fluorescent sialidase substrate. BTP-Neu5Ac can visualize sialidase activities sensitively and selectively in acute rat brain slices. Cancer cells implanted orthotopically in mouse colons and human colon cancers (stages T3-T4) were also clearly detected with BTP-Neu5Ac. The results suggest that BTP-Neu5Ac is useful for histochemical imaging of sialidase activities.
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影响因子：
5.8
作者：
Oku, Naoto;Yamashita, Mina;Kanazawa, Hiroaki
通讯作者：
Kanazawa, Hiroaki
影响因子：
7.7
作者：
Friedland, S;Soetikno, R;Segall, G
通讯作者：
Segall, G
DOI：
10.1016/j.bbalip.2010.01.002
发表时间：
2010-04-01
影响因子：
4.8
作者：
Du, Zhen-Yu;Ma, Tao;Madsen, Lise
通讯作者：
Madsen, Lise
影响因子：
5.7
作者：
Minami, Akira;Shimizu, Hirotaka;Suzuki, Takashi
通讯作者：
Suzuki, Takashi
DOI：
10.1007/978-1-4614-3381-1_17
发表时间：
2012-01-01
期刊：
BIOCHEMICAL ROLES OF EUKARYOTIC CELL SURFACE MACROMOLECULES
影响因子：
--
作者：
Miyagi, Taeko;Takahashi, K.;Shiozaki, K.
通讯作者：
Shiozaki, K.