Propofol facilitates excitatory inputs of cerebellar Purkinje cells by depressing molecular layer interneuron activity during sensory information processing in vivo in mice
Propofol facilitates excitatory inputs of cerebellar Purkinje cells by depressing molecular layer interneuron activity during sensory information processing in vivo in mice
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异丙酚通过抑制小鼠体内感觉信息处理过程中的分子层中间神经元活动来促进小脑浦肯野细胞的兴奋性输入
DOI：
10.1097/wnr.0000000000000449
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发表时间：
2015-10
期刊：
影响因子：
1.7
通讯作者：
Qiu De-Lai
中科院分区：
文献类型：
--
作者：
He Yuan-Yuan;Jin Ri;Jin Wen-Zhe;Liu Heng;Chu Chun-Ping;Qiu De-Lai
异丙酚是一种速效镇静催眠药物，广泛用于麻醉的诱导和维持；它对大脑的不同区域有特定的作用，如躯体感觉皮层的感觉信息传递。然而，异丙酚对小脑浦肯野细胞(PC)感觉刺激诱发反应的影响目前尚不清楚。在本研究中，我们用电生理和药理学方法研究了异丙酚对乌拉坦麻醉小鼠小脑PC和分子水平中间神经元(MLI)面部刺激诱发反应的影响。结果表明，小脑表面灌流异丙酚后，γ-氨基丁酸(GABA)能神经递质(P_1)波幅呈剂量依赖性降低，兴奋性反应(N_1)波幅显著升高。异丙酚抑制P_1的IC_(50)为217.3 ·mgr；M。反之，异丙酚(100 ·mgr；M)可抑制MLI的自发活动和触觉诱发反应。此外，阻断GABAA受体活性可阻断异丙酚(300 ；M)对触觉诱发的抑制反应的抑制和感觉刺激诱发的PC放电频率的增加。这些结果表明，异丙酚抑制触觉刺激诱发的MLI的发放，导致小鼠小脑PC区触觉诱发的抑制反应幅度降低和兴奋反应幅度增加。我们的结果提示，异丙酚通过激活GABAA受体来调节小脑皮质PC和MLI的感觉信息处理。
Propofol is a rapid-acting sedative-hypnotic medication that has been widely used for the induction and maintenance of anesthesia; it has specific actions on different areas of the brain, such as sensory information transmission in the somatosensory cortex. However, the effects of propofol on the properties of sensory stimulation-evoked responses in cerebellar Purkinje cells (PCs) are currently unclear. In the present study, we studied the effects of propofol on facial stimulation-evoked responses in cerebellar PCs and molecular level interneurons (MLIs) in urethane-anesthetized mice using electrophysiological and pharmacological methods. Our results showed that cerebellar surface perfusion with propofol induced a decrease in the amplitude of the gamma-aminobutyric acid (GABA)-ergic component (P1) in a dose-dependent manner, but induced a significant increase in the amplitude of the excitatory response (N1). The IC50 of propofol-induced inhibition of P1 was 217.3 &mgr;M. In contrast, propofol (100 &mgr;M) depressed the spontaneous activity and tactile-evoked responses in MLIs. In addition, blocking GABAA receptor activity abolished the propofol (300 &mgr;M)-induced inhibition of the tactile-evoked inhibitory response and the increase in the sensory stimulation-evoked spike firing rate of PCs. These results indicated that propofol depressed the tactile stimulation-evoked spike firing of MLIs, resulting in a decrease in the amplitude of the tactile-evoked inhibitory response and an increase in the amplitude of the excitatory response in the cerebellar PCs of mice. Our results suggest that propofol modulates sensory information processing in cerebellar cortical PCs and MLIs through the activation of GABAA receptors.
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影响因子：
3.7
作者：
Li Z;Liu X;Zhang Y;Shi J;Zhang Y;Xie P;Yu T
通讯作者：
Yu T
影响因子：
2.9
作者：
Hanrahan SJ;Greger B;Parker RA;Ogura T;Obara S;Egan TD;House PA
通讯作者：
House PA
体内小鼠小脑皮质 Crus II 浦肯野细胞中触觉刺激引起的突触反应
影响因子：
3.7
作者：
Chu CP;Bing YH;Liu QR;Qiu DL
通讯作者：
Qiu DL
影响因子：
3.3
作者：
Qiqing Shi;X. Sun;H. Fang
通讯作者：
Qiqing Shi;X. Sun;H. Fang
分子层中间神经元在小鼠小脑皮质 Crus II 体内感觉信息处理中的作用
影响因子：
3.7
作者：
Chu CP;Bing YH;Liu H;Qiu DL
通讯作者：
Qiu DL