Complete genome sequence of avian paramyxovirus-3 strain Wisconsin: evidence for the existence of subgroups within the serotype.
Complete genome sequence of avian paramyxovirus-3 strain Wisconsin: evidence for the existence of subgroups within the serotype.
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DOI：
10.1016/j.virusres.2009.12.015
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发表时间：
2010-04
期刊：
影响因子：
5
通讯作者：
Samal, Siba K.
中科院分区：
文献类型：
--
作者：
Kumar, Sachin;Nayak, Baibaswata;Samuel, Arthur S.;Xiao, Sa;Collins, Peter L.;Samal, Siba K.
测定了禽副粘病毒（APMV）血清3型威斯康星州株的全基因组序列。该基因组全长16,182个核苷酸（nt），由6个非重叠基因组成，顺序为3 '-N-P/V/W-M-F-HN-L-5'，其3 '端有一个55 nt的前导序列，5'端有一个681 nt的尾随序列。APMV-3株威斯康星州nt和聚集预测的氨基酸（aa）序列与APMV-3株荷兰的核苷酸序列的比较分别显示67%和78%的同一性。两种APMV-3毒株之间的nt和aa序列同一性低于人呼吸道合胞病毒的两个抗原亚组之间的同一性（分别为81和88%）和人偏肺病毒的两个亚组之间的同一性（分别为80和90%）。使用鸡感染后血清进行的相互交叉血凝抑制和交叉中和试验表明，菌株威斯康星州和荷兰在抗原性上高度相关，同源和异源菌株之间的抗体反应性仅存在2 - 4倍差异。两者合计，我们的研究结果表明，两个APMV-3株代表一个单一的血清型与两个亚组，基本上不同的基础上的nt和aa序列，但只有适度的抗原差异。
The complete consensus genome sequence was determined for avian paramyxovirus (APMV) serotype 3 strain Wisconsin. The genome is 16,182 nucleotides (nt) in length, consisting of six non-overlapping genes in the order of 3’-N-P/V/W-M-F-HN-L-5’, with a 55-nt leader at its 3’ end and a 681-nt trailer at its 5’ end. Comparison of the APMV-3 strain Wisconsin nt and the aggregate predicted amino acid (aa) sequences with those of APMV-3 strain Netherlands revealed 67 and 78%, identity, respectively. The nt and aa sequence identities between the two APMV-3 strains were lower than between the two antigenic subgroups of human respiratory syncytial virus (81 and 88% identity, respectively) and the two subgroups of human metapeumovirus (80 and 90% identity, respectively). Reciprocal cross-hemagglutination inhibition and cross-neutralization assays using post infection sera from chickens indicated that strains Wisconsin and Netherlands are highly related antigenically, with only a 2- to 4-fold difference in antibody reactivity between the homologous and heterologous strains. Taken together, our results indicate that the two APMV-3 strains represent a single serotype with two subgroups that differ substantially based on nt and aa sequences, but with only a modest antigenic difference.
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