Regulation of gut luminal serotonin by commensal microbiota in mice.
Regulation of gut luminal serotonin by commensal microbiota in mice.
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DOI：
10.1371/journal.pone.0180745
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sudo N
中科院分区：
文献类型：
--
作者：
Hata T;Asano Y;Yoshihara K;Kimura-Todani T;Miyata N;Zhang XT;Takakura S;Aiba Y;Koga Y;Sudo N
肠腔血清素（5-羟色胺：5-HT）有助于几种胃肠道功能，如蠕动反射。5-HT从肠嗜铬（EC）细胞释放，以响应许多刺激，包括来自肠道微生物群的信号。然而，肠道微生物群调节肠腔中5-HT水平的具体机制尚未阐明。我们以前的工作与gnotobiotic小鼠表明，游离的儿茶酚胺可以产生的共轭的儿茶酚胺的解共轭，因此，我们推测，解共轭的细菌酶可能是肠道微生物可以产生游离的5-HT在肠腔的机制之一。在这项研究中，我们使用无菌（GF）小鼠和无特定病原体（SPF）粪便植物群（EX-GF）感染的无菌小鼠来验证这一假设。GF小鼠盲肠和结肠腔中的5-HT水平显著低于EX-GF小鼠。此外，这些水平在暴露于SPF微生物群后仅3天内迅速增加。在EX-GF小鼠中，大部分5-HT为非结合的游离形式，而在GF小鼠中发现约50%的5-HT为结合形式。这些结果进一步支持了目前的观点，即肠道微生物群在促进具有生物活性的游离5-HT的产生方面起着至关重要的作用。葡萄糖醛酸苷结合的5-HT通过细菌酶解结合可能是导致肠腔中游离5-HT产生的机制之一。
Gut lumen serotonin (5-hydroxytryptamine: 5-HT) contributes to several gastrointestinal functions such as peristaltic reflexes. 5-HT is released from enterochromaffin (EC) cells in response to a number of stimuli, including signals from the gut microbiota. However, the specific mechanism by which the gut microbiota regulates 5-HT levels in the gut lumen has not yet been clarified. Our previous work with gnotobiotic mice showed that free catecholamines can be produced by the deconjugation of conjugated catecholamines; hence, we speculated that deconjugation by bacterial enzymes may be one of the mechanisms whereby gut microbes can produce free 5-HT in the gut lumen. In this study, we tested this hypothesis using germ-free (GF) mice and gnotobiotic mice recolonized with specific pathogen-free (SPF) fecal flora (EX-GF). The 5-HT levels in the lumens of the cecum and colon were significantly lower in the GF mice than in the EX-GF mice. Moreover, these levels were rapidly increased, within only 3 days after exposure to SPF microbiota. The majority of 5-HT was in an unconjugated, free form in the EX-GF mice, whereas approximately 50% of the 5-HT was found in the conjugated form in the GF mice. These results further support the current view that the gut microbiota plays a crucial role in promoting the production of biologically active, free 5-HT. The deconjugation of glucuronide-conjugated 5-HT by bacterial enzymes is likely one of the mechanisms contributing to free 5-HT production in the gut lumen.
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DOI：
10.1152/ajpgi.2001.280.5.g787
发表时间：
2001-05-01
影响因子：
4.5
作者：
Kirkup, AJ;Brunsden, AM;Grundy, D
通讯作者：
Grundy, D
DOI：
10.1152/ajpgi.00090.2004
发表时间：
2004-11-01
影响因子：
4.5
作者：
O'Hara, JR;Ho, WN;Sharkey, KA
通讯作者：
Sharkey, KA
DOI：
10.1097/med.0b013e32835bc703
发表时间：
2013-02
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Gershon MD
通讯作者：
Gershon MD
影响因子：
4.8
作者：
Reigstad, Christopher S.;Salmonson, Charles E.;Kashyap, Purna C.
通讯作者：
Kashyap, Purna C.
影响因子：
3.5
作者：
Bertrand, PP
通讯作者：
Bertrand, PP