Echocardiographic assessment of Xenopus tropicalis heart regeneration.
Echocardiographic assessment of Xenopus tropicalis heart regeneration.
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DOI：
10.1186/s13578-023-00982-z
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发表时间：
2023-02-13
影响因子：
7.5
通讯作者：
Cai D
中科院分区：
文献类型：
--
作者：
Lv L;Guo W;Guan W;Chen Y;Huang R;Yuan Z;Pu Q;Feng S;Zheng X;Li Y;Xiao L;Zhao H;Qi X;Cai D
最近，有报道称，成年X。Tropicalis心脏可以在通过心尖切除的损伤后以几乎无瘢痕的方式再生。因此，成年X。tropicalis提供了一个强大的工具，重演一个完美的再生现象，阐明了心脏再生的基本分子机制，在一个成年人的心脏，并制定了干预策略，在成人心脏的再生，这可能是更适用于哺乳动物比在物种进化程度较低。然而，尚未建立一种无创、快速、实时的方法来观察和测量活体再生心脏的长期动态变化，以监测和评估该模型中的再生和修复状态。本文探讨了超声心动图对X线损伤后形态、解剖结构及心功能的评价方法。通过心尖切除建立了Tropicalis心脏。这项研究的结果首次证明了小动物超声心动图分析可以用于评估X线再生。热带假单胞菌损伤的心脏通过形态学和心功能的恢复，以无瘢痕的完全再生或有粘连的不完全再生的方式再生。小动物超声心动图是一种可靠的、无创的、快速的实时观察和评价X线损伤后再生的长期动态变化的方法。热带心脏在线版本包含补充材料，可通过10.1186/s13578-023-00982-z获得。
Recently, it was reported that the adult X. tropicalis heart can regenerate in a nearly scar-free manner after injury via apical resection. Thus, a cardiac regeneration model in adult X. tropicalis provides a powerful tool for recapitulating a perfect regeneration phenomenon, elucidating the underlying molecular mechanisms of cardiac regeneration in an adult heart, and developing an interventional strategy for the improvement in the regeneration of an adult heart, which may be more applicable in mammals than in species with a lower degree of evolution. However, a noninvasive and rapid real-time method that can observe and measure the long-term dynamic change in the regenerated heart in living organisms to monitor and assess the regeneration and repair status in this model has not yet been established. In the present study, the methodology of echocardiographic assessment to characterize the morphology, anatomic structure and cardiac function of injured X. tropicalis hearts established by apex resection was established. The findings of this study demonstrated for the first time that small animal echocardiographic analysis can be used to assess the regeneration of X. tropicalis damaged heart in a scar-free perfect regeneration or nonperfect regeneration with adhesion manner via recovery of morphology and cardiac function. Small animal echocardiography is a reliable, noninvasive and rapid real-time method for observing and assessing the long-term dynamic changes in the regeneration of injured X. tropicalis hearts. The online version contains supplementary material available at 10.1186/s13578-023-00982-z.
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影响因子：
7.2
作者：
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DOI：
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期刊：
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影响因子：
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影响因子：
3
作者：
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DOI：
10.3791/1738
发表时间：
2010-05-28
期刊：
Journal of visualized experiments : JoVE
影响因子：
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作者：
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通讯作者：
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