Reproductive experience alters neural and behavioural responses to acute oestrogen receptor α activation.
Reproductive experience alters neural and behavioural responses to acute oestrogen receptor α activation.
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DOI：
10.1111/jne.12113
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发表时间：
2013-12
影响因子：
3.2
通讯作者：
Bridges RS
中科院分区：
文献类型：
--
作者：
Byrnes EM;Casey K;Carini LM;Bridges RS
生殖经历（即分娩和哺乳）导致受发情周期影响的焦虑样行为的持续改变。我们最近发现，反复给药选择性雌激素受体(ER)α激动剂丙基吡唑三醇（PPT）会导致初产（但不是未产）雌性大鼠在升高的正迷宫（EPM）上产生焦虑样行为。本研究检测了急性给药PPT对初产和年龄匹配、未生育雌性大鼠EPM行为的影响。此外，在EPM试验后或家兔笼对照中，分别测量室旁核（PVN）和杏仁核中皮质酮分泌、促肾上腺皮质激素释放激素（CRH）基因表达和即时早期基因产物Fos的表达。急性PPT管理显著改变了EPM行为作为生殖经验的功能，未生育的雌性倾向于增加焦虑样行为，而初产的雌性倾向于减少焦虑样行为。在家笼对照中，PPT增加了所有雌性的皮质酮分泌；然而，与未分娩的雌性相比，经载体和ppt治疗的初产雌性的皮质酮水平都降低了。PPT对PVN内CRH mRNA有显著影响，但仅在EPM测试的初产女性中观察到。PPT还增加了epm暴露雌性PVN内Fos的表达；然而，与未分娩的雌性相比，经载体和ppt处理的雌性初产小鼠Fos表达均降低。在杏仁核中，PPT增加了中央杏仁核的Fos免疫反应活性，但没有增加内侧或基底外侧杏仁核的Fos免疫反应活性，尽管这些影响仅在家养笼雌性中观察到。此外，与无药对照相比，在家鼠笼中，无论是用载药还是用ppt处理，初产雌性杏仁核中央核的Fos都有所增加。总的来说，这些数据表明生殖经历改变了对急性ERα激活的行为反应。此外，研究结果表明，下丘脑-肾上腺-垂体轴的中枢调节因生殖经验而改变。
Reproductive experience (i.e. parturition and lactation) leads to persistent alterations in anxietylike behaviour that are influenced by the oestrous cycle. We recently found that repeated administration of the selective oestrogen receptors (ER)α agonist propyl-pyrazole triol (PPT) results in anxiolytic-like behaviours on the elevated plus maze (EPM) in primiparous (but not nulliparous) female rats. The present study examined the effects of the acute administration of PPT on EPM behaviour in primiparous and aged-matched, nulliparous female rats. In addition, corticosterone secretion, corticotrophin-releasing hormone (CRH) gene expression and expression of the immediate early gene product Fos in the paraventricular nucleus (PVN) and amygdala were measured either after EPM testing or in home cage controls. Acute PPT administration significantly modified EPM behaviour as a function of reproductive experience, with nulliparous females tending toward increased anxiety-like behaviours and primiparous females tending toward decreased anxiety-like behaviours. In home cage controls, PPT increased corticosterone secretion in all females; however, both vehicle- and PPT-treated, primiparous females had reduced corticosterone levels compared to their nulliparous counterparts. Significant effects of PPT on CRH mRNA within the PVN were observed after the administration of PPT but only in primiparous females tested on the EPM. PPT also increased Fos expression within the PVN of EPM-exposed females; however, both vehicle- and PPT-treated primiparous females had reduced Fos expression compared to nulliparous females. In the amygdala, PPT increased Fos immunore-activity in the central but not the medial or basolateral amygdala, although these effects were only observed in home cage females. Additionally, both vehicle- and PPT-treated home cage, primiparous females had increased Fos in the central nucleus of the amygdala compared to nullip-arous controls. Overall, these data demonstrate that reproductive experience alters the behavioural response to acute ERα activation. Moreover, the findings suggest that central regulation of the hypothalamic-adrenal-pituitary axis is modified as a consequence of reproductive experience.
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影响因子：
3.2
作者：
Byrnes EM;Babb JA;Bridges RS
通讯作者：
Bridges RS
影响因子：
3.7
作者：
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作者：
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通讯作者：
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作者：
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作者：
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