Pulsed electromagnetic fields promote repair of focal articular cartilage defects with engineered osteochondral constructs.
Pulsed electromagnetic fields promote repair of focal articular cartilage defects with engineered osteochondral constructs.
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脉冲电磁场促进局部关节软骨缺损与工程化骨软骨结构的修复。
DOI：
10.1002/bit.27287
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发表时间：
2020-05
影响因子：
3.8
通讯作者：
Hung CT
中科院分区：
文献类型：
--
作者：
Stefani RM;Barbosa S;Tan AR;Setti S;Stoker AM;Ateshian GA;Cadossi R;Vunjak-Novakovic G;Aaron RK;Cook JL;Bulinski JC;Hung CT
关节软骨损伤是关节疼痛和功能障碍的常见原因。我们假设脉冲电磁场（PEMFs）将以时间和方向依赖的方式改善组织工程软骨移植物的生长和愈合。工程化软骨结构的PEMF刺激首先使用包埋在琼脂糖水凝胶支架中的传代成年犬软骨细胞在体外进行评价。与垂直PEMF（p=0.026）和对照（p=0.012）相比，平行于关节面定向的PEMF线圈诱导了更好的上级修复刚度。与对照组相比，这与平行和垂直PEMF方向的GAG沉积增加相关（分别为p=0.010和0.028）。体外优化后，在初步的体内临床前成年犬模型中评价了PEMF的潜在临床转化。将工程化的骨软骨构建体（106mm X 6mm厚，失活的骨基底）培养至成熟并植入后膝（膝）关节中产生的局灶性缺损中。为了评估加速早期修复，在3个月恢复期后对动物进行评估，微骨折修复作为额外的临床对照。在体内，PEMF导致工程结构中正常软骨细胞（OR：2.5，p=0.051）和蛋白聚糖（OR：5.0，p=0.013）组织学评分的可能性更大。有趣的是，无论PEMF治疗如何，工程化结构在临床评分中优于微骨折（p<0.05）。总的来说，这些研究提供了PEMF刺激增强工程软骨生长和修复的证据，证明了一种潜在的低成本，低风险，非侵入性治疗方式，可加速早期软骨修复。
Articular cartilage injuries are a common source of joint pain and dysfunction. We hypothesized that pulsed electromagnetic fields (PEMFs) would improve growth and healing of tissue engineered cartilage grafts in a time- and direction-dependent manner. PEMF stimulation of engineered cartilage constructs was first evaluated in vitro using passaged adult canine chondrocytes embedded in an agarose hydrogel scaffold. PEMF coils oriented parallel to the articular surface induced superior repair stiffness compared to both perpendicular PEMF (p=0.026) and control (p=0.012). This was correlated with increased GAG deposition in both parallel and perpendicular PEMF orientations compared to control (p=0.010 and 0.028, respectively). Following in vitro optimization, the potential clinical translation of PEMF was evaluated in a preliminary in vivo preclinical adult canine model. Engineered osteochondral constructs (∅ 6 mm x 6 mm thick, devitalized bone base) were cultured to maturity and implanted into focal defects created in the stifle (knee) joint. To assess expedited early repair, animals were assessed after a 3-month recovery period, with microfracture repairs serving as an additional clinical control. In vivo, PEMF led to a greater likelihood of normal chondrocyte (OR: 2.5, p=0.051) and proteoglycan (OR: 5.0, p=0.013) histological scores in engineered constructs. Interestingly, engineered constructs outperformed microfracture in clinical scoring, regardless of PEMF treatment (p<0.05). Overall, the studies provided evidence that PEMF stimulation enhanced engineered cartilage growth and repair, demonstrating a potential low-cost, low-risk, non-invasive treatment modality for expediting early cartilage repair.
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影响因子：
3.8
作者：
Benazzo, Francesco;Zanon, Giacomo;Massari, Leo
通讯作者：
Massari, Leo
影响因子：
2.8
作者：
Chang EY;Pallante-Kichura AL;Bae WC;Du J;Statum S;Wolfson T;Gamst AC;Cory E;Amiel D;Bugbee WD;Sah RL;Chung CB
通讯作者：
Chung CB
影响因子：
7.5
作者：
Fini, Milena;Torricelli, Paola;Giardino, Roberto
通讯作者：
Giardino, Roberto
影响因子：
7
作者：
Cook, J. L.;Kuroki, K.;Lafeber, F. P. J. G.
通讯作者：
Lafeber, F. P. J. G.
影响因子：
2.7
作者：
Cadossi, Matteo;Buda, Roberto Emanuele;Giannini, Sandro
通讯作者：
Giannini, Sandro