Fluorescence-Detected Mid-Infrared Photothermal Microscopy.
Fluorescence-Detected Mid-Infrared Photothermal Microscopy.
复制标题
DOI：
10.1021/jacs.1c03642
复制
发表时间：
2021-08-04
影响因子：
15
通讯作者：
Cheng JX
中科院分区：
文献类型：
--
作者：
Zhang Y;Zong H;Zong C;Tan Y;Zhang M;Zhan Y;Cheng JX
中红外光热显微镜是一种利用可见光束感知脉冲红外激光引起的光热效应的新型化学成像技术。该技术提供了亚微米空间分辨率的红外光谱信息，使活细胞和生物体的红外光谱和成像成为可能。然而，目前中红外光热成像的灵敏度散射对温度的依赖性较弱，散射产生的散斑容易影响成像质量。在这里，我们提出了一种新版本的中红外光热显微镜，其中热敏荧光探针被利用来感知中红外光热效应。荧光强度可以在每开尔文1%的水平上调制，是散射强度调制的100倍。此外，荧光发射不受干扰，从而大大提高了图像质量。此外，荧光团可以靶向特定的细胞器或生物分子，从而增强了光热成像的特异性。光谱保真度是通过对单个细菌进行指纹鉴定来确定的。最后，通过开发一种宽视场荧光检测的中红外光热显微镜，成功地解决了光漂白问题，该显微镜允许对生物标本进行视频速率键选择成像。
Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at submicrometer spatial resolution and enables infrared spectroscopy and imaging of living cells and organisms. Yet, current mid-infrared photothermal imaging sensitivity suffers from a weak dependence of scattering on the temperature, and the image quality is vulnerable to the speckles caused by scattering. Here, we present a novel version of mid-infrared photothermal microscopy in which thermosensitive fluorescent probes are harnessed to sense the mid-infrared photothermal effect. The fluorescence intensity can be modulated at the level of 1% per Kelvin, which is 100 times larger than the modulation of scattering intensity. In addition, fluorescence emission is free of interference, thus much improving the image quality. Moreover, fluorophores can target specific organelles or biomolecules, thus augmenting the specificity of photothermal imaging. Spectral fidelity is confirmed through fingerprinting a single bacterium. Finally, the photobleaching issue is successfully addressed through the development of a wide-field fluorescence-detected mid-infrared photothermal microscope which allows video rate bond-selective imaging of biological specimens.
登录
查看更多内容
影响因子：
19.4
作者：
Liao, Chien-Sheng;Slipchenko, Mikhail N.;Cheng, Ji-Xin
通讯作者：
Cheng, Ji-Xin
影响因子：
48
作者：
Hu, Fanghao;Shi, Lixue;Min, Wei
通讯作者：
Min, Wei
通过拉曼集成中红外光热显微镜对活细胞进行指纹识别
影响因子：
7.4
作者：
Li, Xiaojie;Zhang, Delong;Cheng, Ji-Xin
通讯作者：
Cheng, Ji-Xin
影响因子：
5.7
作者：
Lim, Jong Min;Park, Chanjong;Cho, Minhaeng
通讯作者：
Cho, Minhaeng
影响因子：
16.6
作者：
Du J;Su Y;Qian C;Yuan D;Miao K;Lee D;Ng AHC;Wijker RS;Ribas A;Levine RD;Heath JR;Wei L
通讯作者：
Wei L