Epigenetic Variance, Performing Cooperative Structure with Genetics, Is Associated with Leaf Shape Traits in Widely Distributed Populations of Ornamental Tree Prunus mume.
Epigenetic Variance, Performing Cooperative Structure with Genetics, Is Associated with Leaf Shape Traits in Widely Distributed Populations of Ornamental Tree Prunus mume.
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DOI：
10.3389/fpls.2018.00041
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Ma K;Sun L;Cheng T;Pan H;Wang J;Zhang Q
越来越多的证据表明表观遗传学在表型变异中起着重要作用。然而，人们对重要观赏树梅梅的表观遗传变异知之甚少。我们使用扩增片段长度多态性（AFLP）和甲基化敏感扩增多态性（MSAP）技术以及关联分析和测序来研究表观遗传变异及其与遗传变异、环境因素和性状的关系。通过叶片取样，检测到96份梅花的相对总甲基化水平（29.80%）。相对半甲基化水平（15.77%）高于相对全甲基化水平（14.03%）。表观遗传多样性（I*=0.575，h*=0.393）高于遗传多样性（I=0.484，h=0.319）。在中国西南和东南部地区，栽培种群比野生种群表现出更大的表观遗传多样性。我们发现表观遗传方差和遗传方差以及环境因素分别执行合作结构。特别是，叶长、宽度和面积与相对全甲基化水平和总甲基化水平呈正相关，表明DNA甲基化水平在性状变异中发挥作用。总共检测到 203 个 AFLP 和 423 个 MSAP 相关标记，并对其中 68 个进行了测序。同源分析和功能预测表明，候选标记连锁基因对于叶片形态发育和代谢至关重要，这意味着这些标记在叶片长度、宽度、面积和长宽比的建立中发挥着关键作用。
Increasing evidence shows that epigenetics plays an important role in phenotypic variance. However, little is known about epigenetic variation in the important ornamental tree Prunus mume. We used amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) techniques, and association analysis and sequencing to investigate epigenetic variation and its relationships with genetic variance, environment factors, and traits. By performing leaf sampling, the relative total methylation level (29.80%) was detected in 96 accessions of P. mume. And the relative hemi-methylation level (15.77%) was higher than the relative full methylation level (14.03%). The epigenetic diversity (I∗ = 0.575, h∗ = 0.393) was higher than the genetic diversity (I = 0.484, h = 0.319). The cultivated population displayed greater epigenetic diversity than the wild populations in both southwest and southeast China. We found that epigenetic variance and genetic variance, and environmental factors performed cooperative structures, respectively. In particular, leaf length, width and area were positively correlated with relative full methylation level and total methylation level, indicating that the DNA methylation level played a role in trait variation. In total, 203 AFLP and 423 MSAP associated markers were detected and 68 of them were sequenced. Homologous analysis and functional prediction suggested that the candidate marker-linked genes were essential for leaf morphology development and metabolism, implying that these markers play critical roles in the establishment of leaf length, width, area, and ratio of length to width.
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影响因子：
9.5
作者：
Feng S;Jacobsen SE
通讯作者：
Jacobsen SE
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
通过综合数量性状位点（QTL）分析和关联研究揭示杨属生长性状的遗传结构
影响因子：
9.4
作者：
Du, Qingzhang;Gong, Chenrui;Zhang, Deqiang
通讯作者：
Zhang, Deqiang
影响因子：
3.3
作者：
DeGiorgio, Michael;Jankovic, Ivana;Rosenberg, Noah A.
通讯作者：
Rosenberg, Noah A.
影响因子：
11.6
作者：
Evans, Matthew M. S.
通讯作者：
Evans, Matthew M. S.