Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke.
Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke.
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DOI：
10.1038/s41467-022-29017-w
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发表时间：
2022-03-15
影响因子：
16.6
通讯作者：
George PM
中科院分区：
文献类型：
--
作者：
Oh B;Santhanam S;Azadian M;Swaminathan V;Lee AG;McConnell KW;Levinson A;Song S;Patel JJ;Gardner EE;George PM
中风是世界范围内长期残疾的主要原因，这加剧了对有效康复治疗的需求。干细胞是一种有前途的中风治疗方法，但创造理想的治疗条件是必不可少的。在这里，我们开发了一种导电聚合物系统，用于动物干细胞输送和电调制。使用该系统，人类干细胞移植的电调节改善啮齿动物的功能性中风恢复。增加的内源性干细胞产生对应于改善的功能。转录组分析确定斯钙素2（STC 2）作为最显着上调的基因之一，通过电刺激。慢病毒在移植干细胞中上调和下调STC 2表明，这种糖蛋白是电调节所见的功能改善中的重要介质。此外，脑室内施用重组STC 2中风后赋予功能的好处。总之，我们的导电聚合物系统使干细胞的电调节成为改善恢复和识别重要治疗靶点的潜在方法。Paul乔治及其同事开发了一种导电聚合物系统，使干细胞输送和体内电调制成为可能。采用该系统改善了啮齿动物的功能性中风恢复，并确定了重要的修复途径。
Stroke is a leading cause of long-term disability worldwide, intensifying the need for effective recovery therapies. Stem cells are a promising stroke therapeutic, but creating ideal conditions for treatment is essential. Here we developed a conductive polymer system for stem cell delivery and electrical modulation in animals. Using this system, electrical modulation of human stem cell transplants improve functional stroke recovery in rodents. Increased endogenous stem cell production corresponds with improved function. Transcriptome analysis identified stanniocalcin 2 (STC2) as one of the genes most significantly upregulated by electrical stimulation. Lentiviral upregulation and downregulation of STC2 in the transplanted stem cells demonstrate that this glycoprotein is an essential mediator in the functional improvements seen with electrical modulation. Moreover, intraventricular administration of recombinant STC2 post-stroke confers functional benefits. In summation, our conductive polymer system enables electrical modulation of stem cells as a potential method to improve recovery and identify important therapeutic targets. Paul George and colleagues developed a conductive polymer system to enable stem cell delivery and electrical modulation in vivo. Employing this system improved functional stroke recovery in rodents and identified important repair pathways.
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影响因子：
3.7
作者：
Chang KA;Kim JW;Kim JA;Lee SE;Kim S;Suh WH;Kim HS;Kwon S;Kim SJ;Suh YH
通讯作者：
Suh YH
影响因子：
--
作者：
Fazio EN;Dimattia GE;Chadi SA;Kernohan KD;Pin CL
通讯作者：
Pin CL
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
影响因子：
8.3
作者：
GARCIA, JH;WAGNER, S;HU, XJ
通讯作者：
HU, XJ
影响因子：
16.2
作者：
Bergmann, Olaf;Liebl, Jakob;Frisen, Jonas
通讯作者：
Frisen, Jonas