Tomato brown rugose fruit virus resistance generated by quadruple knockout of homologs of TOBAMOVIRUS MULTIPLICATION1 in tomato.
Tomato brown rugose fruit virus resistance generated by quadruple knockout of homologs of TOBAMOVIRUS MULTIPLICATION1 in tomato.
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DOI：
10.1093/plphys/kiac103
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发表时间：
2022-06-01
期刊：
影响因子：
7.4
通讯作者：
Ishibashi, Kazuhiro
中科院分区：
文献类型：
--
作者：
Ishikawa, Masayuki;Yoshida, Tetsuya;Matsuyama, Momoko;Kouzai, Yusuke;Kano, Akihito;Ishibashi, Kazuhiro
番茄褐皱果病毒（Tomato brown rugose fruit virus，ToBRFV）是烟草花叶病毒属（Tobamovirus）的一种新病毒。ToBRFV克服了埃博拉病毒的抗性基因Tm-22，并在世界范围内迅速传播。ToBRFV抗性的遗传资源是迫切需要的。在这里，我们表明，成簇的规则间隔短回文重复序列/CRISPR相关蛋白9（CRISPR/Cas9）介导的靶向诱变的四个番茄（番茄）同源物的烟草花叶病毒增殖1（TOM 1），拟南芥（拟南芥）基因必不可少的烟草花叶病毒增殖，赋予番茄植物对ToBRFV的抗性。四突变体植物没有表现出可检测到的ToBRFV外壳蛋白（CP）的积累或明显的缺陷，在生长或果实生产。当任何三个的四个TOM 1同源物被破坏，ToBRFV CP积累是可检测的，但大大减少。在三重突变体中，ToBRFV CP积累受到最强烈的抑制，突变体病毒能够在突变体植物中更有效地增殖。然而，这些突变体病毒没有感染四重突变体植物，表明四重突变体植物的抗性是高度持久的。四重突变体植物也表现出对其他三种病毒的抗性。因此，通过CRISPR/Cas9介导的多重基因组编辑可以产生对包括ToBRFV在内的番茄病毒具有强抗性的番茄植物。基因组编辑的植物可以促进ToBRFV抗性番茄育种。编辑番茄中的宿主易感基因赋予了对能够克服当前可用抗性基因的新出现病毒的强抗性。
Tomato brown rugose fruit virus (ToBRFV) is an emerging virus of the genus Tobamovirus. ToBRFV overcomes the tobamovirus resistance gene Tm-22 and is rapidly spreading worldwide. Genetic resources for ToBRFV resistance are urgently needed. Here, we show that clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9)-mediated targeted mutagenesis of four tomato (Solanum lycopersicum) homologs of TOBAMOVIRUS MULTIPLICATION1 (TOM1), an Arabidopsis (Arabidopsis thaliana) gene essential for tobamovirus multiplication, confers resistance to ToBRFV in tomato plants. Quadruple-mutant plants did not show detectable ToBRFV coat protein (CP) accumulation or obvious defects in growth or fruit production. When any three of the four TOM1 homologs were disrupted, ToBRFV CP accumulation was detectable but greatly reduced. In the triple mutant, in which ToBRFV CP accumulation was most strongly suppressed, mutant viruses capable of more efficient multiplication in the mutant plants emerged. However, these mutant viruses did not infect the quadruple-mutant plants, suggesting that the resistance of the quadruple-mutant plants is highly durable. The quadruple-mutant plants also showed resistance to three other tobamovirus species. Therefore, tomato plants with strong resistance to tobamoviruses, including ToBRFV, can be generated by CRISPR/Cas9-mediated multiplexed genome editing. The genome-edited plants could facilitate ToBRFV-resistant tomato breeding. Editing of host susceptibility genes in tomato confers strong resistance against an emerging virus capable of overcoming currently available resistance genes.
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影响因子：
16.6
作者：
Ritter A;Iñigo S;Fernández-Calvo P;Heyndrickx KS;Dhondt S;Shi H;De Milde L;Vanden Bossche R;De Clercq R;Eeckhout D;Ron M;Somers DE;Inzé D;Gevaert K;De Jaeger G;Vandepoele K;Pauwels L;Goossens A
通讯作者：
Goossens A
影响因子：
13.8
作者：
Gomez MA;Lin ZD;Moll T;Chauhan RD;Hayden L;Renninger K;Beyene G;Taylor NJ;Carrington JC;Staskawicz BJ;Bart RS
通讯作者：
Bart RS
影响因子：
5.6
作者：
Pramanik D;Shelake RM;Park J;Kim MJ;Hwang I;Park Y;Kim JY
通讯作者：
Kim JY
对植物病毒的隐性抗性：超出翻译起始因子的潜在抗性基因。
影响因子：
5.2
作者：
Hashimoto M;Neriya Y;Yamaji Y;Namba S
通讯作者：
Namba S
DOI：
10.3390/v7072778
发表时间：
2015-06-24
期刊：
Viruses
影响因子：
--
作者：
Sanfaçon H
通讯作者：
Sanfaçon H