Cell-scale dynamic recycling and cortical flow of the actin-myosin cytoskeleton for rapid cell migration.
Cell-scale dynamic recycling and cortical flow of the actin-myosin cytoskeleton for rapid cell migration.
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DOI：
10.1242/bio.20122899
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发表时间：
2013-02-15
期刊：
影响因子：
2.4
通讯作者：
Tsujioka M
中科院分区：
文献类型：
--
作者：
Yumura S;Itoh G;Kikuta Y;Kikuchi T;Kitanishi-Yumura T;Tsujioka M
肌动蛋白和肌球蛋白II在细胞迁移中起主要作用。而伪足延伸肌动蛋白聚合已被深入研究，很少有人注意到如何其余的肌动蛋白细胞骨架，如肌动蛋白皮质有助于细胞迁移。在这项研究中，皮质肌动蛋白和肌球蛋白II丝同时观察到迁移的网骨细胞下全内反射荧光显微镜。皮质肌动蛋白和肌球蛋白II丝保持静止相对于底层的细胞先进。然而，光漂白实验和直接观察细丝后的荧光恢复表明，它们迅速翻转。当细胞脱离基质时，肌动蛋白和肌球蛋白细丝显示出强烈的逆行流动。因此，当细胞在基质上迁移时，皮质细胞骨架牢固地保持基质以产生动力。目前的研究还表明，肌球蛋白II如何定位到迁移细胞的后部区域。所观察到的肌动蛋白和肌球蛋白II细丝的动态周转有助于它们的亚基在整个细胞中的再循环，并使细胞骨架能够快速重组。
Actin and myosin II play major roles in cell migration. Whereas pseudopod extension by actin polymerization has been intensively researched, less attention has been paid to how the rest of the actin cytoskeleton such as the actin cortex contributes to cell migration. In this study, cortical actin and myosin II filaments were simultaneously observed in migrating Dictyostelium cells under total internal reflection fluorescence microscopy. The cortical actin and myosin II filaments remained stationary with respect to the substratum as the cells advanced. However, fluorescence recovery after photobleaching experiments and direct observation of filaments showed that they rapidly turned over. When the cells were detached from the substratum, the actin and myosin filaments displayed a vigorous retrograde flow. Thus, when the cells migrate on the substratum, the cortical cytoskeleton firmly holds the substratum to generate the motive force instead. The present studies also demonstrate how myosin II localizes to the rear region of the migrating cells. The observed dynamic turnover of actin and myosin II filaments contributes to the recycling of their subunits across the whole cell and enables rapid reorganization of the cytoskeleton.
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影响因子：
64.8
作者：
FORSCHER, P;LIN, CH;THOMPSON, C
通讯作者：
THOMPSON, C
影响因子：
7.8
作者：
Gardel, Margaret L.;Sabass, Benedikt;Ji, Lin;Danuser, Gaudenz;Schwarz, Ulrich S.;Waterman, Clare M.
通讯作者：
Waterman, Clare M.
影响因子：
5.6
作者：
Haviv, Lior;Gillo, David;Bernheim-Groswasser, Anne
通讯作者：
Bernheim-Groswasser, Anne
影响因子：
56.9
作者：
LEE, J;GUSTAFSSON, M;JACOBSON, K
通讯作者：
JACOBSON, K
影响因子：
16.2
作者：
LIN, CH;FORSCHER, P
通讯作者：
FORSCHER, P