Involvement of peripheral ionotropic glutamate receptors in orofacial thermal hyperalgesia in rats.
Involvement of peripheral ionotropic glutamate receptors in orofacial thermal hyperalgesia in rats.
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DOI：
10.1186/1744-8069-7-75
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发表时间：
2011-09-28
期刊：
影响因子：
3.3
通讯作者：
Iwata K
中科院分区：
文献类型：
--
作者：
Honda K;Noma N;Shinoda M;Miyamoto M;Katagiri A;Kita D;Liu MG;Sessle BJ;Yasuda M;Iwata K
本研究的目的是阐明三叉神经尾部亚核（Vc）和上颈脊髓（C1-C2）神经元对注射谷氨酸（Glu）后口面部区域的热或冷刺激敏感的机制。将 Glu 涂在舌头或胡须垫皮肤上会导致 Vc-C2 神经元中的头缩反射和细胞外信号调节激酶 (ERK) 磷酸化增强。在注射Glu的大鼠中，冷刺激舌头而不是胡须垫皮肤后，头缩反射和ERK磷酸化也增强，而热刺激舌头或胡须垫皮肤后，头缩反射和ERK磷酸化也增强。将离子型谷氨酸受体拮抗剂施用到舌头或胡须垫皮肤后，热刺激舌头或胡须垫皮肤后增强的缩头反射和ERK磷酸化以及冷刺激舌头但胡须垫皮肤后的增强的缩头反射和ERK磷酸化均受到抑制。此外，鞘内注射MEK1/2抑制剂PD98059可显着抑制注射Glu的大鼠增强的头缩回反射、舌或须垫皮肤注射Glu的大鼠的热缩头反射以及舌注射Glu的大鼠的冷缩头反射。目前的研究结果表明，外周 Glu 受体机制可能会导致舌头的冷痛觉过敏，但不会导致面部皮肤的冷痛觉过敏，并且还会导致舌头和面部皮肤的热痛觉过敏，并且 Vc-C2 神经元中的丝裂原激活蛋白激酶级联可能参与这些 Glu 诱发的痛觉过敏效应。
The purpose of the present study was to elucidate the mechanisms that may underlie the sensitization of trigeminal spinal subnucleus caudalis (Vc) and upper cervical spinal cord (C1-C2) neurons to heat or cold stimulation of the orofacial region following glutamate (Glu) injection. Glu application to the tongue or whisker pad skin caused an enhancement of head-withdrawal reflex and extracellular signal-regulated kinase (ERK) phosphorylation in Vc-C2 neurons. Head-withdrawal reflex and ERK phosphorylation were also enhanced following cold stimulation of the tongue but not whisker pad skin in Glu-injected rats, and the head-withdrawal reflex and ERK phosphorylation were enhanced following heat stimulation of the tongue or whisker pad skin. The enhanced head-withdrawal reflex and ERK phosphorylation after heat stimulation of the tongue or whisker pad skin, and those following cold stimulation of the tongue but not whisker pad skin were suppressed following ionotropic glutamate receptor antagonists administration into the tongue or whisker pad skin. Furthermore, intrathecal administration of MEK1/2 inhibitor PD98059 caused significant suppression of enhanced head-withdrawal reflex in Glu-injected rats, heat head-withdrawal reflex in the rats with Glu injection into the tongue or whisker pad skin and cold head-withdrawal reflex in the rats with Glu injection into the tongue. The present findings suggest that peripheral Glu receptor mechanisms may contribute to cold hyperalgesia in the tongue but not in the facial skin, and also contribute to heat hyperalgesia in the tongue and facial skin, and that the mitogen-activated protein kinase cascade in Vc-C2 neurons may be involved in these Glu-evoked hyperalgesic effects.
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影响因子：
7.4
作者：
Ge, HY;Madeleine, P;Arendt-Nielsen, L
通讯作者：
Arendt-Nielsen, L
影响因子：
2.5
作者：
Adachi, K.;Murray, G. M.;Sessle, B. J.
通讯作者：
Sessle, B. J.
影响因子：
2.5
作者：
Cairns, BE;Sessle, BJ;Hu, JW
通讯作者：
Hu, JW
影响因子：
3.3
作者：
Kobayashi A;Shinoda M;Sessle BJ;Honda K;Imamura Y;Hitomi S;Tsuboi Y;Okada-Ogawa A;Iwata K
通讯作者：
Iwata K
DOI：
10.3109/07367228709144606
发表时间：
1987-01-01
期刊：
SOMATOSENSORY RESEARCH
影响因子：
--
作者：
GREEN, BG;GELHARD, B
通讯作者：
GELHARD, B