Submicron topography design for controlling staphylococcal bacterial adhesion and biofilm formation.
Submicron topography design for controlling staphylococcal bacterial adhesion and biofilm formation.
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DOI：
10.1002/jbm.a.37369
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发表时间：
2022-06
影响因子：
4.9
通讯作者：
Siedlecki, Christopher A.
中科院分区：
文献类型：
--
作者：
Xu, Li-Chong;Siedlecki, Christopher A.
关键词：
用纳米或微米纹理结构修饰表面形貌是抑制微生物粘附和生物膜形成的有效方法，从而防止生物材料相关感染，而不修饰材料的表面化学/本体性质，并且不引起抗生素抗性。这份手稿的重点是亚微米纹理图案与有序阵列的聚氨酯（PU）生物材料表面的支柱，努力了解表面支柱的功能和表面特性的影响，粘附和定植反应的两个葡萄球菌菌株。设计并在PU膜表面上制备了五种具有不同柱尺寸的亚微米图案，并对葡萄球菌菌株（S。epidermidis RP 62A和S.金黄色葡萄球菌纽曼D2 C）进行了表征。结果表明，所有亚微米纹理表面均显著降低了细菌粘附，抑制了生物膜的形成，且细菌粘附随表面面积分数的降低而线性降低。表面润湿性没有表现出与细菌粘附的线性相关性，表明表面接触面积主导细菌粘附。由此可见，纹理图案的设计应该最小化表面积分数以减少细菌与表面的相互作用，但以确保柱的机械强度的方式，以避免塌陷。这些研究结果可能提供了一个基本原理的聚合物表面的设计，用于医疗器械。
Surface topography modification with nano- or micro- textured structures has been an efficient approach to inhibit microbial adhesion and biofilm formation and thereby to prevent biomaterial associated infection without modification of surface chemistry/bulk properties of materials and without causing antibiotic resistance. This manuscript focuses on submicron textured patterns with ordered arrays of pillars on polyurethane (PU) biomaterial surfaces in an effort to understand the effects of surface pillar features and surface properties on adhesion and colonization responses of two Staphylococcal strains. Five submicron patterns with a variety of pillar dimensions were designed and fabricated on PU film surfaces and bacterial adhesion and biofilm formation of Staphylococcal strains (S. epidermidis RP62A and S. aureus Newman D2C) were characterized. Results show that all submicron textured surface significantly reduced bacterial adhesion and inhibited biofilm formation, and bacterial adhesion linearly decreased with the reduction in top surface area fraction. Surface wettability did not show a linear correlation with bacterial adhesion, suggesting that surface contact area dominates bacterial adhesion. From this, it appears that the design of textured patterns should minimize surface area fraction to reduce the bacterial interaction with surfaces but in a way that ensures the mechanical strength of pillars in order to avoid collapse. These findings may provide a rationale for design of polymer surfaces for antifouling medical devices.
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影响因子：
4.9
作者：
Milner, KR;Snyder, AJ;Siedlecki, CA
通讯作者：
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影响因子：
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影响因子：
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影响因子：
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