Mass spectrometric imaging for biomedical tissue analysis.
Mass spectrometric imaging for biomedical tissue analysis.
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DOI：
10.1021/cr100012c
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发表时间：
2010-05-12
期刊：
影响因子：
62.1
通讯作者：
Heeren, Ron M. A.
中科院分区：
文献类型：
--
作者：
Chughtai, Kamila;Heeren, Ron M. A.
质谱仪被描述为世界上使用过的最小的称重秤。1质谱（MS）是一种独特的技术，具有跨学科的性质，可以自由地跨越物理学，化学和生物学的边界。质谱是一种伟大的科学工具，因为它能够确定大生物分子复合物，单个生物分子，小有机分子和单个原子及其同位素的质量。从世纪的第一个十年发明以来，质谱法在灵敏度、分辨率和质量范围方面经历了巨大的改进。它目前在所有科学学科中得到应用，如化学，物理学，生物学，药理学，医学，生物化学和生物农业。在20世纪80年代引入的“软”电离源，如Fenn等人的电喷雾电离（ESI）2和Karas等人的基质辅助激光解吸/电离（MALDI）3，彻底改变了质谱法，因为它提供了分析大的完整生物分子的能力。因此，MS成为生物科学不可替代的工具。ESI和MALDI的发展使得更小的生物分子（如药物和代谢物）以及更大的生物分子（如脂质、肽甚至蛋白质）的电离成为可能。2，4我们在本综述中使用的分子量（Mw）范围定义如下。低Mw范围包括1至500 Da的元素和分子。Mw在500至2000 Da之间的分子属于中等Mw范围。Mw> 2000 Da的所有分子被认为是高分子量类别的一部分。它超出了本次审查的范围，以涵盖MS的所有发展。相反，本次审查的重点是MS，即质谱成像（MSI）的最新，快速发展的创新之一。这种年轻的技术得益于过去几十年来MS的所有方法和技术发展。在过去的20年里，MSI已经从一个深奥的，专业的技术研究，只有少数研究人员的技术，现在发现自己在主流MS的中心舞台在过去的几年里，该技术已经成熟，找到应用在许多不同的领域，与仪器的发展采取了所有MS仪器制造商导致在这一领域的研究小组的数量迅速上升活跃。因此，对这一领域进行彻底审查是及时的，也是必要的，以便为所有外地新来者提供一个起点。在本文中，我们描述和审查约20年的MSI发展的角度来看，其应用于生物医学成像。我们将强调关键的研究步骤和陷阱，
A mass spectrometer is described as the smallest weighing scale ever used in the world. 1 Mass spectrometry (MS) is a unique technique that has an interdisciplinary nature, which freely crosses the borders of physics, chemistry, and biology. Mass spectrometry is a great scientific tool due to its capabilities to determine the mass of large biomolecular complexes, individual biomolecules, small organic molecules, and single atoms and their isotopes. Right from the time of its invention in the first decade of the 20th century, mass spectrometry has undergone tremendous improvements in terms of its sensitivity, resolution, and mass range. It currently finds applications in all scientific disciplines, such as chemistry, physics, biology, pharmacology, medicine, biochemistry, and bioagro-based industry. Introduction of “soft” ionization sources such as electrospray ionization (ESI) by Fenn et al. 2 and matrix-assisted laser desorption/ionization (MALDI) by Karas et al. 3 in the 1980s revolutionized mass spectrometry, as it offered the capability to analyze large intact biomolecules. As such, MS became an irreplaceable tool for the biological sciences. The development of both ESI and MALDI made possible the ionization of smaller biomolecules such as drugs and metabolites as well larger biomolecules such as lipids, peptides, and even proteins. 2, 4 The molecular weight (Mw) ranges we use in this review are defined as follows. The low Mw range includes elements and molecules from 1 to 500 Da. Molecules with Mw between 500 and 2000 Da fall in the medium Mw range. All molecules with Mw> 2000 Da are considered to be part of the high molecular weight class. It goes beyond the scope of this review to cover all developments in MS. Rather this review focuses on one of the latest, rapidly developing innovations in MS, namely mass spectrometric imaging (MSI). This young technique takes benefit from all methodological and technological developments in general MS over the last decades. Over the last 20 years MSI has transformed from an esoteric, specialist technology studied by few researchers only to a technique that now finds itself at the center stage of mainstream MS. Over the last years the technology has matured to find applications in many different areas, with instrument developments taken up by all MS instrument manufacturers resulting in a rapid rise of the number of research groups active in this area. A thorough review of the area is therefore timely and needed to offer a starting point for all newcomers to the field. In this paper we describe and review approximately 20 years of MSI development from the perspective of its application to biomedical imaging. We will emphasize the key research steps and pitfalls that determine
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影响因子：
4.3
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