The type III secretion system is involved in the invasion and intracellular survival of Escherichia coli K1 in human brain microvascular endothelial cells.
The type III secretion system is involved in the invasion and intracellular survival of Escherichia coli K1 in human brain microvascular endothelial cells.
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DOI：
10.1111/j.1574-6968.2009.01763.x
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发表时间：
2009-11
影响因子：
2.1
通讯作者：
Kim KS
中科院分区：
文献类型：
--
作者：
Yao Y;Xie Y;Perace D;Zhong Y;Lu J;Tao J;Guo X;Kim KS
III型分泌系统已在许多革兰氏阴性菌中被记录，包括肠出血性大肠杆菌。我们先前已经证明了在脑膜炎致病性大肠杆菌中存在一个假定的III型分泌系统。coliK1菌株，简称E.大肠杆菌III型分泌2（ETT 2）。ETT2基因在脑膜炎致病E. coli K1菌株EC10（O7：K1）的ETT2基因组序列分析表明，ETT2含有epr、epa和eiv基因，但存在突变、缺失和插入。我们构建了ETT2或eivA基因缺失的EC 10突变体，并在人脑微血管内皮细胞中评估了它们对细菌致病性的贡献。ETT 2缺失突变体在侵袭和细胞内存活方面与亲本E. coliK1菌株EC10。ETT 2中eiv A缺失的突变株在人脑微血管内皮细胞中的侵袭和细胞内存活也明显缺陷，并且通过反式互补使eiv突变株的缺陷恢复到亲本菌株EC 10的水平。提示ETT2在大肠杆菌的发病机制中起一定作用。大肠杆菌K1感染，包括脑膜炎。
Type III secretion systems have been documented in many Gram-negative bacteria, including enterohemorrhagic Escherichia coli. We have previously shown the existence of a putative type III secretion system in meningitis-causing E. coli K1 strains, referred to as E. coli type III secretion 2 (ETT2). The sequence of ETT2 in meningitis-causing E. coli K1 strain EC10 (O7:K1) revealed that ETT2 comprises the epr, epa and eiv genes, but bears mutations, deletions and insertions. We constructed the EC10 mutants deleted of ETT2 or eivA gene, and their contributions to bacterial pathogenesis were evaluated in human brain microvascular endothelial cells. The deletion mutant of ETT2 exhibited defects in invasion and intracellular survival compared to the parental E. coli K1 strain EC10. The mutant deleted of eivA within ETT2 was also significantly defective in invasion and intracellular survival in human brain microvascular endothelial cells, and the defects of the eiv mutant were restored to the levels of the parent strain EC10 by trans-complementation. These findings suggest that ETT2 plays a role in the pathogenesis of E. coli K1 infection, including meningitis.
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DOI：
10.1038/nrmicro1952
发表时间：
2008-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.6
作者：
Fields, KA;Mead, DJ;Hackstadt, T
通讯作者：
Hackstadt, T
影响因子：
15.9
作者：
Lee, CA
通讯作者：
Lee, CA
影响因子：
3.1
作者：
Stins, MF;Nemani, PV;Kim, KS
通讯作者：
Kim, KS
影响因子：
3.4
作者：
Kim, KJ;Elliott, SJ;Kim, KS
通讯作者：
Kim, KS