Evaluating the pharmacokinetics and in vivo cancer targeting capability of Au nanocages by positron emission tomography imaging.
Evaluating the pharmacokinetics and in vivo cancer targeting capability of Au nanocages by positron emission tomography imaging.
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DOI：
10.1021/nn300464r
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发表时间：
2012-07-24
期刊：
影响因子：
17.1
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Wang Y;Liu Y;Luehmann H;Xia X;Brown P;Jarreau C;Welch M;Xia Y
近年来，金纳米笼作为一种新型的光热传感器和癌症治疗的药物载体而出现。然而，由于缺乏快速可靠的体内分布成像方式，它们的药代动力学和肿瘤靶向能力在很大程度上仍未被探索。本文制备了具有可控理化性质的金纳米笼，并用具有高比活性的64Cu进行放射性标记，并利用正电子发射断层扫描（PET）对其进行体内评价。我们对飞摩尔给药的药代动力学研究表明，30纳米的纳米笼比55纳米的纳米笼具有更好的生物分布特征，同时具有更高的血液潴留和更低的肝脏和脾脏摄取。在小鼠EMT-6乳腺癌模型中，小笼子也显示出明显高于大笼子的肿瘤摄取水平和肿瘤与肌肉的比例。定量PET成像证实了金纳米笼在肿瘤内的快速积聚和滞留。直接快速成像金纳米笼在体内分布的能力使我们能够进一步优化其物理化学性质，用于一系列治疗应用。
Gold nanocages have recently emerged as a novel class of photothermal transducers and drug carriers for cancer treatment. However, their pharmacokinetics and tumor targeting capability remain to be largely unexplored due to the lack of an imaging modality for quick and reliable mapping of their distributions in vivo. Herein, Au nanocages were prepared with controlled physicochemical properties and radiolabeled with 64Cu in high specific activities for in vivo evaluation using positron emission tomography (PET). Our pharmacokinetic studies with femtomolar administrations suggest that nanocages of 30 nm in size had a greatly improved biodistribution profile than nanocages of 55 nm in size, together with higher blood retention and lower hepatic and splenic uptakes. In a murine EMT-6 breast cancer model, the small cages also showed a significantly higher level of tumor uptake and a greater tumor-to-muscle ratio than the large cages. Quantitative PET imaging confirmed rapid accumulation and retention of Au nanocages inside the tumors. The ability to directly and quickly image the distribution of Au nanocages in vivo allows us to further optimize their physicochemical properties for a range of theranostic applications.
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影响因子：
15
作者：
Moon, Geon Dae;Choi, Sung-Wook;Cai, Xin;Li, Weiyang;Cho, Eun Chul;Jeong, Unyong;Wang, Lihong V.;Xia, Younan
通讯作者：
Xia, Younan
影响因子：
3.7
作者：
Holback, Hillary;Yeo, Yoon
通讯作者：
Yeo, Yoon
影响因子：
18.3
作者：
Ng, Kenneth K.;Lovell, Jonathan F.;Zheng, Gang
通讯作者：
Zheng, Gang
影响因子：
10.8
作者：
Chen, Jingyi;Wang, Danling;Li, Xingde
通讯作者：
Li, Xingde
影响因子：
11.2
作者：
Nielsen, Carsten H.;Kimura, Richard H.;Gambhir, Sanjiv S.
通讯作者：
Gambhir, Sanjiv S.