An Atherogenic Diet Disturbs Aquaporin 5 Expression in Liver and Adipocyte Tissues of Apolipoprotein E-Deficient Mice: New Insights into an Old Model of Experimental Atherosclerosis.
An Atherogenic Diet Disturbs Aquaporin 5 Expression in Liver and Adipocyte Tissues of Apolipoprotein E-Deficient Mice: New Insights into an Old Model of Experimental Atherosclerosis.
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致动脉粥样硬化饮食干扰载脂蛋白E缺乏小鼠肝脏和脂肪细胞组织中水通道蛋白5的表达：对实验性动脉粥样硬化旧模型的新认识。
DOI：
10.3390/biomedicines9020150
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发表时间：
2021-02-04
期刊：
影响因子：
4.7
通讯作者：
Castro R
中科院分区：
文献类型：
--
作者：
da Silva IV;Whalen CA;Mattie FJ;Florindo C;Huang NK;Heil SG;Neuberger T;Ross AC;Soveral G;Castro R
血管内皮细胞的功能障碍与动脉粥样硬化和心血管疾病的发病机制密切相关，而心血管疾病是全球主要的死亡原因。水通道蛋白（AQP）是一种促进水和甘油跨细胞膜转运的膜通道，近年来被发现与心血管系统的稳态有关。载脂蛋白E缺乏（apoE−/−）小鼠是研究动脉粥样硬化进展的常见模型。然而，在这个动脉粥样硬化模型中的水通道蛋白的表达模式的特点很差。在这项研究中，apoE−/−小鼠被喂食致动脉粥样硬化的高脂肪（HF）或对照饮食。在多个时间点采集血浆以评估代谢紊乱。在终点，使用高场磁共振成像定量主动脉粥样硬化负荷。此外，在肝脏、白色脂肪细胞组织（WAT）和棕色脂肪细胞组织（BAT）中评估了几种AQP亚型的转录水平。结果显示，与对照组相比，HF喂养的小鼠呈现出加重的全身炎症和动脉粥样硬化表型，在全身甲基化状态、循环氨基酸或血浆总谷胱甘肽方面没有重大差异。此外，与对照组相比，在HF喂养小鼠的所有研究组织中检测到亚型AQP 5的过表达。这些结果表明，AQP 5在饮食诱导的动脉粥样硬化中的新作用值得进一步研究。
The dysfunction of vascular endothelial cells is profoundly implicated in the pathogenesis of atherosclerosis and cardiovascular disease, the global leading cause of death. Aquaporins (AQPs) are membrane channels that facilitate water and glycerol transport across cellular membranes recently implicated in the homeostasis of the cardiovascular system. Apolipoprotein-E deficient (apoE−/−) mice are a common model to study the progression of atherosclerosis. Nevertheless, the pattern of expression of AQPs in this atheroprone model is poorly characterized. In this study, apoE−/− mice were fed an atherogenic high-fat (HF) or a control diet. Plasma was collected at multiple time points to assess metabolic disturbances. At the endpoint, the aortic atherosclerotic burden was quantified using high field magnetic resonance imaging. Moreover, the transcriptional levels of several AQP isoforms were evaluated in the liver, white adipocyte tissue (WAT), and brown adipocyte tissue (BAT). The results revealed that HF-fed mice, when compared to controls, presented an exacerbated systemic inflammation and atherosclerotic phenotype, with no major differences in systemic methylation status, circulating amino acids, or plasma total glutathione. Moreover, an overexpression of the isoform AQP5 was detected in all studied tissues from HF-fed mice when compared to controls. These results suggest a novel role for AQP5 on diet-induced atherosclerosis that warrants further investigation.
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影响因子：
5.6
作者：
da Silva, Ines Vieira;Diaz-Saez, Francisco;Soveral, Graca
通讯作者：
Soveral, Graca
影响因子：
1.5
作者：
Grajeda-Iglesias C;Aviram M
通讯作者：
Aviram M
DOI：
10.1007/978-94-024-1057-0_15
发表时间：
2017-01-01
期刊：
AQUAPORINS
影响因子：
--
作者：
da Silva, Ines Vieira;Soveral, Graca
通讯作者：
Soveral, Graca
影响因子：
3.7
作者：
Fontijn RD;Volger OL;van der Pouw-Kraan TC;Doddaballapur A;Leyen T;Baggen JM;Boon RA;Horrevoets AJ
通讯作者：
Horrevoets AJ
影响因子：
3.9
作者：
da Silva, Ines Vieira;Cardoso, Carlos;Soveral, Graca
通讯作者：
Soveral, Graca