Serum protein profiles suggest a possible link between qi deficiency constitution and Pi-qi-deficiency syndrome of chronic superficial gastritis
Serum protein profiles suggest a possible link between qi deficiency constitution and Pi-qi-deficiency syndrome of chronic superficial gastritis
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血清蛋白谱提示气虚体质与慢性浅表性胃炎脾气虚证之间可能存在联系
DOI：
10.1016/j.jtcms.2019.11.004
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发表时间：
2019-10
影响因子：
--
通讯作者：
Anlong Xu
中科院分区：
文献类型：
--
作者：
Xinhui Gao;Leiming You;Aijie Liu;Xiaopu Sang;Ting’an Li;Shen Zhang;Kunyu Li;Guangrui Huang;Ting Wang;Anlong Xu
目的探讨慢性浅表性胃炎（CSG）气虚质与脾气虚证之间的联系。采用无标记定量蛋白质组学方法，对两个病例群体血清样品中的差异表达蛋白质（DEPs）进行鉴定：病例人群1（QDC受试者）和病例人群2（CSG PQDS患者）。分析了在两个病例人群中发现的DEP，以确定共同的DEP作为参与QDC和PQDS之间联系的蛋白质的潜在候选者。基于京都基因和基因组途径百科全书（KEGG）和基因本体论（GO）富集分析和蛋白质-蛋白质相互作用网络的分析，我们评估了这些潜在的血清candides.ResultsWe发现24和28蛋白的差异表达的情况下，人口1和2，分别与对照人口。系统聚类分析表明，同一群体的DEPs表达谱聚为一类，而不同群体的DEPs表达谱则呈分离状态。此外，GO分析揭示了两个病例人群共同的10种DEP主要与细胞代谢和免疫系统过程的负调节相关，而KEGG分析表明这些蛋白质与补体和凝血级联反应以及过氧化物酶体增殖物激活受体信号传导相关。值得注意的是，血清中C4 b结合蛋白β链，糖基磷脂酰肌醇特异性磷脂酶D1和MS-F1轻链可变区蛋白的水平显着高于两个病例人群相比，特别是在CSG与PQDS.ConclusionThe结果在这里提供了新的见解PQDS的CSG从QDC发展的分子机制，并为将来在中西医结合中的应用提供候选血清生物标志物。
ObjectiveTo identify potential serum protein candidates involved in linking the traditional Chinese medicine (TCM)-defined qi deficiency constitution (QDC) to Pi-qi-deficiency syndrome (PQDS) of chronic superficial gastritis (CSG).MethodsUsing participants with the TCM-defined balanced constitution as a control population, label-free quantitative proteomics was adopted to identify differentially expressed proteins (DEPs) in serum samples from two case populations: case population 1 (participants with QDC) and case population 2 (patients with PQDS of CSG). The DEPs discovered in both case populations were analyzed to identify common DEPs as potential candidates for proteins involved in the link between QDC and PQDS. Based on Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and Gene Ontology (GO) enrichment analysis and analysis of protein–protein interaction networks, we evaluated the possible functions of these potential serum candidates.ResultsWe discovered 24 and 28 proteins that were differentially expressed in case populations 1 and 2, respectively, compared with the control population. Hierarchical clustering analysis showed that the expression profile of DEPs of individuals from the same population clustered well, while those from different populations were segregated. Furthermore, GO analysis revealed the 10 DEPs that were common to both case populations to be mainly associated with negative regulation of cellular metabolic and immune system processes while KEGG analysis indicated these proteins to be associated with complement and coagulation cascades and peroxisome proliferator-activated receptor signaling. Notably, serum levels of C4b-binding protein beta chain, glycosylphosphatidylinositol-specific phospholipase D1 and MS-F1 light chain variable region proteins were notably higher in the two case populations compared with the control, particularly in the case of CSG with PQDS.ConclusionThe results presented here provide new insights into the molecular mechanisms underlying development of PQDS of CSG from QDC, and suggest candidate serum biomarkers for future application in integrative medicine.
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影响因子：
3.4
作者：
Qu Z;Gao F;Li L;Zhang Y;Jiang Y;Yu L;Zhou Y;Zheng H;Tong W;Li G;Tong G
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Tong G
DOI：
10.1084/jem.148.1.207
发表时间：
1978-07-01
期刊：
The Journal of experimental medicine
影响因子：
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通讯作者：
Nussenzweig V
DOI：
--
发表时间：
2005-04
期刊：
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
影响因子：
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作者：
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Yun-Jian Luo;Zong-Chang Xiu;Sui-Ping Huang
DOI：
--
发表时间：
1990-08
期刊：
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Yang Cb;Tang Fk;Pan Xz
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Yang Cb;Tang Fk;Pan Xz
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影响因子：
5.3
作者：
Zhu Q;Wu N;Liu G;Zhou Y;Liu S;Chen J;Liu J;Zuo Y;Liu Z;Chen W;Chen Y;Chen J;Lin M;Zhao Y;Yang Y;Wang S;Yang X;Ma Y;Wang J;Chen X;Zhang J;Shen J;Wu Z;Qiu G
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Qiu G