Label-Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain.
Label-Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain.
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高致病性猪繁殖与呼吸综合征病毒及其弱毒株感染肺泡巨噬细胞差异表达膜蛋白的无标记定量蛋白质组学分析
DOI：
10.1002/pmic.201700101
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发表时间：
2017-12
期刊：
影响因子：
3.4
通讯作者：
Tong G
中科院分区：
文献类型：
--
作者：
Qu Z;Gao F;Li L;Zhang Y;Jiang Y;Yu L;Zhou Y;Zheng H;Tong W;Li G;Tong G
高致病性猪繁殖与呼吸综合征病毒（PRRSV）与其弱毒株感染宿主细胞的能力存在显著差异。不同毒力菌株侵入宿主细胞的机制仍然相对未知。在这项研究中，肺泡巨噬细胞（PAM）感染HP-PRRSV（HuN 4）和AP-PRRSV（HuN 4-F112）24小时，然后收获并进行无标记定量MS。共鉴定了2849种蛋白质，包括95种差异表达的蛋白质。其中，26个蛋白质位于膜上。差异表达最多的蛋白质涉及对刺激的应答、代谢过程和免疫系统过程，主要具有结合和催化活性的功能。通过串分析一起评估分化簇CD 163、波形蛋白（Vim）和nm II以及检测到的蛋白质，其阐明了潜在不同的感染机制。根据功能注释，不同毒力的PRRSV主要在免疫、炎症、免疫逃避以及细胞凋亡等方面存在差异。这是首次尝试从膜蛋白方面探讨HP-PRRSV及其减毒株感染PAM的差异特征，这将有助于进一步了解HP-PRRSV和AP-PRRSV不同的感染机制。
Significant differences exist between the highly pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) and its attenuated pathogenic (AP) strain in the ability to infect host cells. The mechanisms by which different virulent strains invade host cells remain relatively unknown. In this study, pulmonary alveolar macrophages (PAMs) are infected with HP‐PRRSV (HuN4) and AP‐PRRSV (HuN4‐F112) for 24 h, then harvested and subjected to label‐free quantitative MS. A total of 2849 proteins are identified, including 95 that are differentially expressed. Among them, 26 proteins are located on the membrane. The most differentially expressed proteins are involved in response to stimulus, metabolic process, and immune system process, which mainly have the function of binding and catalytic activity. Cluster of differentiation CD163, vimentin (VIM), and nmII as well as detected proteins are assessed together by string analysis, which elucidated a potentially different infection mechanism. According to the function annotations, PRRSV with different virulence may mainly differ in immunology, inflammation, immune evasion as well as cell apoptosis. This is the first attempt to explore the differential characteristics between HP‐PRRSV and its attenuated PRRSV infected PAMs focusing on membrane proteins which will be of great help to further understand the different infective mechanisms of HP‐PRRSV and AP‐PRRSV.
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DOI：
10.1083/jcb.201309092
发表时间：
2014-04-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bays JL;Peng X;Tolbert CE;Guilluy C;Angell AE;Pan Y;Superfine R;Burridge K;DeMali KA
通讯作者：
DeMali KA
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影响因子：
4.6
作者：
Gao J;Xiao S;Xiao Y;Wang X;Zhang C;Zhao Q;Nan Y;Huang B;Liu H;Liu N;Lv J;Du T;Sun Y;Mu Y;Wang G;Syed SF;Zhang G;Hiscox JA;Goodfellow I;Zhou EM
通讯作者：
Zhou EM
影响因子：
3.3
作者：
Jiang, Yi-feng;Xia, Tian-qi;Tong, Guang-zhi
通讯作者：
Tong, Guang-zhi
影响因子：
4.8
作者：
Chen, ZG;Zhang, XL;Ni, F
通讯作者：
Ni, F
DOI：
10.1007/978-1-4614-7651-1_1
发表时间：
2013-01-01
期刊：
VIRAL ENTRY INTO HOST CELLS
影响因子：
--
作者：
Jolly, Clare L.;Sattentau, Quentin J.
通讯作者：
Sattentau, Quentin J.