Functional roles of LaeA, polyketide synthase, and glucose oxidase in the regulation of ochratoxin A biosynthesis and virulence in Aspergillus carbonarius.
Functional roles of LaeA, polyketide synthase, and glucose oxidase in the regulation of ochratoxin A biosynthesis and virulence in Aspergillus carbonarius.
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DOI：
10.1111/mpp.13013
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发表时间：
2021-01
影响因子：
4.9
通讯作者：
Sionov E
中科院分区：
文献类型：
--
作者：
Maor U;Barda O;Sadhasivam S;Bi Y;Levin E;Zakin V;Prusky DB;Sionov E
在曲霉菌种类中，炭黑曲霉是赭曲霉毒素A （OTA）的主要生产者，但这种次生代谢物对真菌毒力的贡献尚未得到评估。我们鉴定了三种因子在赤霉素OTA合成和致病性调控中的功能和作用：LaeA，一种调控次生代谢物产生的转录因子；OTA生物合成所需的聚酮合成酶；和葡萄糖氧化酶（GOX），在真菌生长过程中调节葡萄糖酸（GLA）的积累和宿主组织的酸化。在炭黑田鼠中缺失laeA导致定殖油桃和葡萄的OTA产量显著降低。∆laeA突变体不能有效地酸化定植组织，这是GLA产量减少的直接结果，导致与野生型（WT）相比，受感染果实的毒力减弱。设计的Acpks基因敲除突变体在离体和定植果实中完全抑制了OTA的产生。有趣的是，生理分析显示，∆Acpks突变体的定殖模式与WT菌株相似，定殖组织中GLA的产量很高，这表明OTA积累与炭黑弧菌的致病性无关。Acgox基因的破坏使赤霞珠产生GLA失活，与WT菌株相比，该突变体在受感染果实中的毒力减弱。这些数据表明，全球调节因子LaeA和GOX是通过控制真菌次级代谢和感染重要基因的转录来调节赤霉素致病性的关键因素。LaeA和GOX是通过调控真菌次生代谢和侵染重要基因的转录而调控赤霉病果实致病性的关键因子；OTA对炭疽弧菌毒力没有贡献。
Aspergillus carbonarius is the major producer of ochratoxin A (OTA) among Aspergillus species, but the contribution of this secondary metabolite to fungal virulence has not been assessed. We characterized the functions and addressed the roles of three factors in the regulation of OTA synthesis and pathogenicity in A. carbonarius: LaeA, a transcriptional factor regulating the production of secondary metabolites; polyketide synthase, required for OTA biosynthesis; and glucose oxidase (GOX), regulating gluconic acid (GLA) accumulation and acidification of the host tissue during fungal growth. Deletion of laeA in A. carbonarius resulted in significantly reduced OTA production in colonized nectarines and grapes. The ∆laeA mutant was unable to efficiently acidify the colonized tissue, as a direct result of diminished GLA production, leading to attenuated virulence in infected fruit compared to the wild type (WT). The designed Acpks‐knockout mutant resulted in complete inhibition of OTA production in vitro and in colonized fruit. Interestingly, physiological analysis revealed that the colonization pattern of the ∆Acpks mutant was similar to that of the WT strain, with high production of GLA in the colonized tissue, suggesting that OTA accumulation does not contribute to A. carbonarius pathogenicity. Disruption of the Acgox gene inactivated GLA production in A. carbonarius, and this mutant showed attenuated virulence in infected fruit compared to the WT strain. These data identify the global regulator LaeA and GOX as critical factors modulating A. carbonarius pathogenicity by controlling transcription of genes important for fungal secondary metabolism and infection. LaeA and GOX are critical factors modulating A. carbonarius pathogenicity in fruits by controlling transcription of genes important for fungal secondary metabolism and infection; OTA does not contribute to A. carbonarius virulence.
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影响因子：
5.6
作者：
Kumar D;Tannous J;Sionov E;Keller N;Prusky D
通讯作者：
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影响因子：
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DOI：
10.1016/j.ijfoodmicro.2017.12.028
发表时间：
2018-03-02
影响因子：
5.4
作者：
Gil-Serna, Jessica;Garcia-Diaz, Marta;Patino, Belen
通讯作者：
Patino, Belen
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2.5
作者：
BUSSINK, HJD;BUXTON, FP;VISSER, J
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VISSER, J