Convergent evolution of sodium ion selectivity in metazoan neuronal signaling.
Convergent evolution of sodium ion selectivity in metazoan neuronal signaling.
复制标题
DOI：
10.1016/j.celrep.2012.06.016
复制
发表时间：
2012-08-30
期刊：
影响因子：
8.8
通讯作者：
Moran Y
中科院分区：
文献类型：
--
作者：
Gur Barzilai M;Reitzel AM;Kraus JE;Gordon D;Technau U;Gurevitz M;Moran Y
后生动物电压门控钠离子通道的离子选择性是神经元信号传递的关键，长期以来一直被认为是由四个保守的氨基酸组成的环，它们构成了通道孔处的离子选择性过滤器(SF)。然而，除了偏爱钙离子的通道外，海葵Nematostella vetensis的Na+通道同源物的表达和特征表明，它是一种具有非规范SF的钠选择性通道。诱变和生理分析表明，除SF外的孔道元素决定了该通道对Na+的偏好。系统发育分析将线虫的Na+选择性通道归入一个独有的通道群，该通道群在5.4亿年前从钙离子传导的Na+通道同源物中分离出来。对钠离子选择性离子通道的鉴定不同于双侧动物的离子通道，表明钠离子在神经元信号转导中的选择性在这两个动物谱系中独立出现。传导钙离子的►钠离子通道同系物出现在10亿多年前，►钠离子选择性在蛇床草和比拉特里亚分别进化。►钠离子选择性是由通道孔的结构差异决定的。在这个通道中，钠的选择性是由选择性过滤器和不同于高等动物(Bilateria)的孔结构所赋予的。系统发育分析表明，在5.4亿年前，刺螺(海葵、珊瑚和水母)的钠选择性与Bilateria>；的进化是独立的，反映了在不断变化的环境中改善神经元信号的需求。
Ion selectivity of metazoan voltage-gated Na+ channels is critical for neuronal signaling and has long been attributed to a ring of four conserved amino acids that constitute the ion selectivity filter (SF) at the channel pore. Yet, in addition to channels with a preference for Ca2+ ions, the expression and characterization of Na+ channel homologs from the sea anemone Nematostella vectensis, a member of the early-branching metazoan phylum Cnidaria, revealed a sodium-selective channel bearing a noncanonical SF. Mutagenesis and physiological assays suggest that pore elements additional to the SF determine the preference for Na+ in this channel. Phylogenetic analysis assigns the Nematostella Na+-selective channel to a channel group unique to Cnidaria, which diverged >540 million years ago from Ca2+-conducting Na+ channel homologs. The identification of Cnidarian Na+-selective ion channels distinct from the channels of bilaterian animals indicates that selectivity for Na+ in neuronal signaling emerged independently in these two animal lineages. ► Na+ channel homologs that conduct Ca2+ appeared more than a billion years ago ► Na+ selectivity evolved separately in Cnidaria and Bilateria ► Na+ selectivity is conferred by structural differences at the channel pore Moran and colleagues have now characterized sodium channel homologs from a sea anemone, revealing four members with a preference for calcium ion conductance, as well as a sodium-selective channel. Sodium selectivity in this channel is conferred by a selectivity filter and pore structure that differ from those of higher animals (Bilateria). A phylogenetic analysis indicates that sodium selectivity in Cnidaria (sea anemones, corals, and jellyfish) evolved independently from that in Bilateria >540 million years ago, reflecting requirements for improved neuronal signaling in a changing environment.
登录
查看更多内容
DOI：
10.1073/pnas.1106363108
发表时间：
2011-05-31
影响因子：
11.1
作者：
Liebeskind, Benjamin J.;Hillis, David M.;Zakon, Harold H.
通讯作者：
Zakon, Harold H.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
3
作者：
Marlow, Heather Q.;Srivastava, Mansi;Martindale, Mark Q.
通讯作者：
Martindale, Mark Q.
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1016/s0006-291x(05)80775-2
发表时间：
1992-07-15
影响因子：
3.1
作者：
SATO, C;MATSUMOTO, G
通讯作者：
MATSUMOTO, G