Sperm exocytosis increases the amount of PH-20 antigen on the surface of guinea pig sperm.
Sperm exocytosis increases the amount of PH-20 antigen on the surface of guinea pig sperm.
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DOI：
10.1083/jcb.103.4.1289
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发表时间：
1986-10
影响因子：
7.8
通讯作者：
MYLES, DG
中科院分区：
文献类型：
--
作者：
COWAN, AE;PRIMAKOFF, P;MYLES, DG
已有证据表明PH-20蛋白在精子粘附到卵细胞透明带中起作用（Primakoff， P.， H. Hyatt, and D. G. Myles, 1985， J. Cell Biol.）。, 101:2239 - 2244)。顶体反应后PH-20蛋白从原表面结构域迁移到新的表面结构域（Myles， D. G. and P. Primakoff, 1984， J. Cell biology .）。, 99:1634 - 1641)。顶体反应是一种胞外活动，导致分泌颗粒膜的一个区域，顶体内膜（IAM）插入到质膜中。顶体反应后，PH-20蛋白从头后表面的初始结构域迁移到IAM。我们现在已经发现了精子表面PH-20蛋白调控的一个新的动态特征；胞吐增加PH-20蛋白的表面表达。顶体反应后，精子表面PH-20抗原位点的数量增加了大约三倍。这些新的抗原位点通过将IAM插入质膜而显示在细胞膜表面。我们的证据表明，在顶体反应之前，细胞内PH-20抗原群定位于IAM。当阻止PH-20蛋白的表面群体迁移时，顶体反应精子的IAM上仍能检测到PH-20蛋白。间接免疫荧光法也可检测到PH-20蛋白在通透性顶体完整精子的IAM上的表达。因此，精子细胞通过将大约三分之二的蛋白质隔离在细胞膜上，然后通过胞外活动将这些蛋白质暴露在细胞表面，从而调节其表面PH-20蛋白的数量。这可能是调节细胞表面组成的一般机制，其中细胞表面蛋白质的数量需要迅速增加。
Evidence has been presented that the PH-20 protein functions in sperm adhesion to the egg zona pellucida (Primakoff, P., H. Hyatt, and D. G. Myles, 1985, J. Cell Biol., 101:2239-2244). The PH-20 protein migrates from its original surface domain to a new surface domain after the acrosome reaction (Myles, D. G., and P. Primakoff, 1984, J. Cell Biol., 99:1634-1641). The acrosome reaction is an exocytotic event that results in insertion of a region of the secretory granule membrane, the inner acrosomal membrane (IAM), into the plasma membrane. After the acrosome reaction, PH-20 protein migrates to the IAM from its initial domain on the posterior head surface. We have now found a new dynamic feature of the regulation of PH-20 protein on the sperm surface; exocytosis increases the surface expression of PH-20 protein. After the acrosome reaction there is an approximately threefold increase in the number of PH-20 antigenic sites on the sperm surface. These new antigenic sites are revealed on the surface by insertion of the IAM into the plasma membrane. Our evidence indicates that before the acrosome reaction an intracellular population of PH-20 antigen is localized to the IAM. When migration of the surface population of the PH-20 protein is prevented, PH-20 protein can still be detected on the IAM of acrosome-reacted sperm. Also, PH-20 protein can be detected on the IAM of permeabilized acrosome-intact sperm by indirect immunofluorescence. Thus, the sperm cell regulates the amount of PH-20 protein on its surface by sequestering about two-thirds of the protein on an intracellular membrane and subsequently exposing this population on the cell surface by an exocytotic event. This may be a general mechanism for regulating cell surface composition where a rapid increase in the amount of a cell surface protein is required.
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DOI：
10.1002/mrd.1120110205
发表时间：
1985-01-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
HINRICHSEN, AC;TOPFERPETERSEN, E;SCHILL, WB
通讯作者：
SCHILL, WB
影响因子：
64.5
作者：
MYLES, DG;PRIMAKOFF, P;BELLVE, AR
通讯作者：
BELLVE, AR
DOI：
10.1016/0889-1605(85)90113-2
发表时间：
1985-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
OLSON, GE;WINFREY, VP
通讯作者：
WINFREY, VP
影响因子：
7.8
作者：
Staehelin, L A
通讯作者：
Staehelin, L A
影响因子：
2.5
作者：
NODA, YD;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R