(18)F-fluorodeoxyglucose positron emission tomography-computed tomography for assessing organ distribution of stressed red blood cells in mice.
(18)F-fluorodeoxyglucose positron emission tomography-computed tomography for assessing organ distribution of stressed red blood cells in mice.
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DOI：
10.1038/s41598-021-82100-y
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发表时间：
2021-01-28
影响因子：
4.6
通讯作者：
Li N
中科院分区：
文献类型：
--
作者：
Yin WY;Yuan J;Zhang ZM;Mei C;Xu W;Tang YX;Peng F;Li N
红细胞在高温胁迫下的病理状态与衰老或受损的红细胞相似。红细胞可用18f -氟脱氧葡萄糖（FDG）有效标记。本研究的目的是应用18F-FDG PET/CT评估应激红细胞在体内的红细胞吞噬和器官分布。在高温（48℃）下诱导红细胞制备应激红细胞。荧光活化细胞分选（FACS）用于分析红细胞的活性氧（ROS）生成、细胞内Ca2+浓度和膜磷脂酰丝氨酸（PS）外化。18F-FDG用于红细胞标记和红细胞吞噬评估。最后，应用18F-FDG PET/CT显示和测量应激红细胞在小鼠体内的器官分布。与未处理的红细胞相比，应激红细胞的细胞体积减少，ROS水平、细胞内Ca2+浓度和PS暴露增加。红细胞可用18F-FDG标记。通过对FACS和放射性的评估，应激红细胞倾向于被巨噬细胞吞噬。18F-FDG PET/CT成像显示应激红细胞主要被困在脾脏，而未处理的红细胞仍在循环系统中。因此，通过PET/CT评估，应激红细胞可以被18F-FDG有效地标记，并倾向于被困在小鼠的脾脏中。
Red blood cells (RBCs) stressed by high temperature are similar to senescent or damaged RBCs in pathological conditions. RBCs can be efficiently labelled with 18F-fluorodeoxyglucose (FDG). The aim of this study was to assess stressed RBCs erythrophagocytosis and organ distribution in vivo with the application of 18F-FDG PET/CT. RBCs were induced under high temperature (48 °C) to prepare stressed RBCs. Fluorescence-activated cell sorting (FACS) was used to analyse reactive oxygen species (ROS) generation, intracellular Ca2+ concentration and membrane phosphatidylserine (PS) externalization of RBCs. 18F-FDG was used to label RBCs and assess the erythrophagocytosis. Finally, 18F-FDG PET/CT was applied to reveal and measure the organ distribution of stressed RBCs in mice. Compared with untreated RBCs, stressed RBCs decreased in cell volume and increased in ROS level, intracellular Ca2+ concentration, and PS exposure. RBCs could be labelled by 18F-FDG. Stressed RBCs tended to be phagocytosed by macrophages via assessment of FACS and radioactivity. 18F-FDG PET/CT imaging showed that stressed RBCs were mainly trapped in spleen, while untreated RBCs remained in circulation system. Thus, stressed RBCs can be effectively labelled by 18F-FDG and tend to be trapped in spleen of mice as assessed by PET/CT.
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