Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h).
Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h).
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在烟夜蛾子囊病毒 3 h (HvAV-3h) 传播系统中使用 RT-qPCR 选择内参基因进行表达分析
DOI：
10.1038/s41598-017-07684-w
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发表时间：
2017-08-01
影响因子：
4.6
通讯作者：
Wan H
中科院分区：
文献类型：
--
作者：
Chen ZS;Han NN;Li JH;Huang GH;Wan H
Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp Microplitis similis Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: β-actin1 (ACT1), β-actin2 (ACT2), elongation factor 1 (EF1), elongation factor 2 (EF2), ribosomal protein L10 (L10), ribosomal protein L17A (L17A), superoxide dismutase (SOD), 28S ribosome (28S), Tubulin (TUB) and 18S ribosome (18S). The samples were originally from various virus concentrations and points-in-time of experimental treatments using RefFinder and four algorithms. The results showed that EF1 was the most stable internal gene in S. exigua and M. similis and that EF2 was the most stable in the IOZCAS-Spex-II-A cell line, and the stability of reference genes were confirmed via the expression levels of two inhibitor of apoptosis-like (iap-like) genes from Heliothis virescens ascovirus 3 h (HvAV-3h). This study provides a crucial basis for future research that explores the molecular mechanisms of the pathogenesis of ascoviruses.
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烟夜蛾子囊病毒3h(HvAV-3h)感染三种主要农业害虫生长发育比较
影响因子：
3.7
作者：
Li SJ;Wang X;Zhou ZS;Zhu J;Hu J;Zhao YP;Zhou GW;Huang GH
通讯作者：
Huang GH
棉蚜（半翅目：蚜科）qRT-PCR 分析中基因表达数据标准化的参考基因的鉴定和验证
DOI：
10.1093/jisesa/iew003
发表时间：
2016
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Ma KS;Li F;Liang PZ;Chen XW;Liu Y;Gao XW
通讯作者：
Gao XW
影响因子：
2.7
作者：
Dheda, K;Huggett, JF;Zumla, A
通讯作者：
Zumla, A
影响因子：
10.5
作者：
Bideshi, DK;Tan, YP;Federici, BA
通讯作者：
Federici, BA
影响因子：
3.8
作者：
Cheng, XW;Carner, GR;Brown, TM
通讯作者：
Brown, TM