Engineering cryoelectrospun elastin-alginate scaffolds to serve as stromal extracellular matrices.
Engineering cryoelectrospun elastin-alginate scaffolds to serve as stromal extracellular matrices.
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DOI：
10.1088/1758-5090/ac6b34
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发表时间：
2022-05-17
期刊：
影响因子：
9
通讯作者：
Xie, Yubing
中科院分区：
文献类型：
--
作者：
Ramesh, Pujhitha;Moskwa, Nicholas;Hanchon, Zachary;Koplas, Adam;Nelson, Deirdre A.;Mills, Kristen L.;Castracane, James;Larsen, Melinda;Sharfstein, Susan T.;Xie, Yubing
关键词：
模拟感兴趣区域的天然细胞外基质（ECM）的物理、生物化学和机械特性的基于支架的再生策略可以影响细胞生长和功能。现有的ECM模拟支架，包括海绵、海绵、水凝胶和纳米纤维-水凝胶复合材料，不能同时模拟健康软组织ECM的典型组成、形貌、孔径、孔隙率和粘弹性。在这项工作中，我们使用冷冻静电纺丝制造三维多孔支架，具有最小的纤维骨架，孔径和机械性能类似于软组织结缔组织ECM。我们使用唾液腺作为我们的软组织模型，发现脱细胞唾液腺（DSG）基质具有纤维骨架，10-30 μm的孔隙，120 Pa的压痕模量和约200 s的松弛半衰期。我们使用弹性蛋白和藻酸盐作为天然的顺应性生物材料，水作为冷冻电纺支架的溶剂，以模拟DSG的结缔组织ECM的结构和粘弹性。优化工艺参数以生产具有类似于DSG的期望形貌和顺应性的支架，具有>100个支架/运行的高产率。使用水作为溶剂，而不是有机溶剂，是至关重要的，以产生生物相容性支架与理想的地形;此外，它允许一个绿色化学制造过程。在这里，我们证明了冷冻电纺支架支持NIH 3 T3成纤维细胞250至450 μm渗透到支架中，细胞存活和基质细胞表型的维持。因此，我们证明了弹性蛋白-藻酸盐冷冻电纺支架模拟ECM的许多结构和功能特性，并具有将来用于再生医学应用的潜力。
Scaffold-based regenerative strategies that emulate physical, biochemical, and mechanical properties of the native extracellular matrix (ECM) of the region of interest can influence cell growth and function. Existing ECM-mimicking scaffolds, including nanofiber mats, sponges, hydrogels, and nanofiber-hydrogel composites are unable to simultaneously mimic typical composition, topography, pore size, porosity, and viscoelastic properties of healthy soft-tissue ECM. In this work, we used cryoelectrospinning to fabricate 3D porous scaffolds with minimal fibrous backbone, pore size and mechanical properties similar to soft-tissue connective tissue ECM. We used salivary glands as our soft tissue model and found the decellularized salivary gland (DSG) matrix to have a fibrous backbone, 10–30 μm pores, 120 Pa indentation modulus, and ~200 s relaxation half time. We used elastin and alginate as natural, compliant biomaterials and water as the solvent for cryoelectrospinning scaffolds to mimic the structure and viscoelasticity of the connective tissue ECM of the DSG. Process parameters were optimized to produce scaffolds with desirable topography and compliance similar to DSG, with a high yield of >100 scaffolds/run. Using water as solvent, rather than organic solvents, was critical to generate biocompatible scaffolds with desirable topography; further, it permitted a green chemistry fabrication process. Here, we demonstrate that cryoelectrospun scaffolds support penetration of NIH 3T3 fibroblasts 250 to 450 μm into the scaffold, cell survival, and maintenance of a stromal cell phenotype. Thus, we demonstrate that elastin-alginate cryoelectrospun scaffolds mimic many structural and functional properties of ECM and have potential for future use in regenerative medicine applications.
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影响因子：
--
作者：
Gupta SK;Dinda AK;Potdar PD;Mishra NC
通讯作者：
Mishra NC
影响因子：
5.6
作者：
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通讯作者：
Dityatev, Alexander
影响因子：
10
作者：
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通讯作者：
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DOI：
10.1046/j.1365-2818.1997.1940755.x
发表时间：
1997-04-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
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作者：
Braet, F;deZanger, R;Wisse, E
通讯作者：
Wisse, E
DOI：
10.1016/j.matbio.2018.03.023
发表时间：
2018-10
期刊：
Matrix biology : journal of the International Society for Matrix Biology
影响因子：
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作者：
Iozzo RV;Gubbiotti MA
通讯作者：
Gubbiotti MA