Modification of decellularized goat-lung scaffold with chitosan/nanohydroxyapatite composite for bone tissue engineering applications.
Modification of decellularized goat-lung scaffold with chitosan/nanohydroxyapatite composite for bone tissue engineering applications.
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DOI：
10.1155/2013/651945
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发表时间：
2013
影响因子：
--
通讯作者：
Mishra NC
中科院分区：
文献类型：
--
作者：
Gupta SK;Dinda AK;Potdar PD;Mishra NC
从山羊尸体肺组织中提取细胞，制备脱细胞山羊肺支架，并采用壳聚糖/纳米羟基磷灰石复合材料对支架进行改性，用于骨组织工程应用。MTT实验显示，将成骨细胞植入壳聚糖/纳米羟基磷灰石修饰的脱细胞支架上，与未修饰的脱细胞支架相比，细胞生长明显更高。细胞植入支架的SEM分析显示，经过7天的培养，细胞具有良好的粘附性，并且细胞在壳聚糖/纳米羟基磷灰石修饰的脱细胞支架上扩散。在壳聚糖/纳米羟基磷灰石修饰脱细胞支架上生长的成骨细胞中，骨组织特异性骨钙素基因的表达清楚地表明，在壳聚糖/纳米羟基磷灰石修饰脱细胞支架下，细胞保持了成骨表型。综上所述，壳聚糖/纳米羟基磷灰石复合材料修饰脱细胞山羊肺支架具有增强的成骨潜能，可用于骨组织工程支架。
Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue, and the scaffold was modified with chitosan/nanohydroxyapatite composite for the purpose of bone tissue engineering applications. MTT assay with osteoblasts, seeded over the chitosan/nanohydroxyapatite-modified decellularized scaffold, demonstrated significantly higher cell growth as compared to the decellularized scaffold without modification. SEM analysis of cell-seeded scaffold, after incubation for 7 days, represented a good cell adhesion, and the cells spread over the chitosan/nanohydroxyapatite-modified decellularized scaffold. Expression of bone-tissue-specific osteocalcin gene in the osteoblast cells grown over the chitosan/nanohydroxyapatite-modified decellularized scaffold clearly signifies that the cells maintained their osteoblastic phenotype with the chitosan/nanohydroxyapatite-modified decellularized scaffold. Therefore, it can be concluded that the decellularized goat-lung scaffold-modified with chitosan/nanohydroxyapatite composite, may provide enhanced osteogenic potential when used as a scaffold for bone tissue engineering.
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影响因子：
3
作者：
Sharma, Chhavi;Dinda, Amit Kumar;Mishra, Narayan Chandra
通讯作者：
Mishra, Narayan Chandra
影响因子：
14
作者：
Ma, ZW;Kotaki, M;Ramakrishna, S
通讯作者：
Ramakrishna, S
影响因子：
3.6
作者：
Borschel, GH;Dennis, RG;Kuzon, WM
通讯作者：
Kuzon, WM
影响因子：
14
作者：
Damink, LHHO;Dijkstra, PJ;Feijen, J
通讯作者：
Feijen, J
DOI：
10.1016/j.msec.2012.12.015
发表时间：
2013-04-01
影响因子：
7.9
作者：
Gautam, Sneh;Dinda, Amit Kumar;Mishra, Narayan Chandra
通讯作者：
Mishra, Narayan Chandra