The trade-off between the light-harvesting and photoprotective functions of fucoxanthin-chlorophyll proteins dominates light acclimation in Emiliania huxleyi (clone CCMP 1516).

The trade-off between the light-harvesting and photoprotective functions of fucoxanthin-chlorophyll proteins dominates light acclimation in Emiliania huxleyi (clone CCMP 1516).
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岩藻黄质-叶绿素蛋白的光捕获和光保护功能之间的权衡主导着艾米利亚赫胥黎(克隆 CCMP 1516)的光适应。

DOI:
10.1111/nph.12373
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发表时间:
2013
期刊:
The New phytologist
影响因子:
--
通讯作者:
McKew BA
McKew BA
中科院分区:
--
文献类型:
--
作者:
McKew BA

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光适应的成本和效益的机制的理解需要了解植物生理学是如何受到叶绿体分子结构的变化的影响。我们测试了假设的变化,光合作用的光依赖性,非光化学猝灭和PSII光失活引起的叶绿体蛋白质丰度的变化,在Emiliania huxleyi菌株CCMP 1516生长在30(低光; LL)和1000(高光; HL)μmol光子m−2s− 1光子通量密度。适应LL和HL的细胞之间的碳比光饱和总光合速率没有显著差异。适应LL通过增加低光下生物量特定的光吸收和总光合速率而使细胞受益,而适应HL通过降低强光下PSII的光失活速率而使细胞受益。光捕获(Lhcf),光保护(LI 818-like)和光系统II(PSII)核心复合物的相对丰度的差异伴随着生理学的差异:特别是,Lhcf:PSII在LL下更大,而LI 818:PSII在HL中更大。因此,赫胥黎氏的光适应涉及光吸收和光保护特性之间的权衡,这可以归因于PSII光捕获天线中蛋白质丰度和组成的变化。
Mechanistic understanding of the costs and benefits of photoacclimation requires knowledge of how photophysiology is affected by changes in the molecular structure of the chloroplast.We tested the hypothesis that changes in the light dependencies of photosynthesis, nonphotochemical quenching and PSII photoinactivation arises from changes in the abundances of chloroplast proteins inEmiliania huxleyistrain CCMP 1516 grown at 30 (Low Light; LL) and 1000 (High Light; HL) μmol photons m−2s−1photon flux densities.Carbon‐specific light‐saturated gross photosynthesis rates were not significantly different between cells acclimated to LL and HL. Acclimation to LL benefited cells by increasing biomass‐specific light absorption and gross photosynthesis rates under low light, whereas acclimation to HL benefited cells by reducing the rate of photoinactivation of PSII under high light. Differences in the relative abundances of proteins assigned to light‐harvesting (Lhcf), photoprotection (LI818‐like), and the photosystem II (PSII) core complex accompanied differences in photophysiology: specifically, Lhcf:PSII was greater under LL, whereas LI818:PSII was greater in HL.Thus, photoacclimation inE. huxleyiinvolved a trade‐off amongst the characteristics of light absorption and photoprotection, which could be attributed to changes in the abundance and composition of proteins in the light‐harvesting antenna of PSII.
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