Spatial Patterns and Age-Related Changes of the Collagen Crimp in the Human Cornea and Sclera.
Spatial Patterns and Age-Related Changes of the Collagen Crimp in the Human Cornea and Sclera.
复制标题
人角膜和巩膜中胶原蛋白压接的空间模式和年龄相关的变化。
DOI：
10.1167/iovs.17-23474
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发表时间：
2018-06-01
影响因子：
4.4
通讯作者：
Sigal IA
中科院分区：
文献类型：
--
作者：
Gogola A;Jan NJ;Brazile B;Lam P;Lathrop KL;Chan KC;Sigal IA
胶原蛋白是眼睛的主要承重成分，胶原蛋白卷曲是组织机械行为的关键决定因素。我们测试的假设，胶原蛋白卷曲形态不同的人角膜和巩膜，并随着年龄的增长。我们分析了20名人类供体的20只正常眼睛的42个轴向全眼球切片，年龄从0.08（1个月）到97岁不等。使用偏振光显微镜对切片进行成像，以获得来自两个角膜和六个巩膜区域的μ m级纤维束/薄片取向。通过波纹度、弯曲度和振幅对预置形态进行定量。全眼球中位波度、弯曲度和振幅分别为0.127弧度、1.002和0.273 μm。然而，这些参数在地球仪上并不一致，而是呈现出不同的、一致的模式。所有压接参数均随年龄增长而显著降低，不同区域之间的年龄相关降低差异显著。利姆布斯的卷曲形态随年龄变化最剧烈，新生儿的卷曲最大，老年人的卷曲最小。眼内压相关的卷曲参数降低可能是年龄相关的巩膜和角膜硬化的潜在机制之一，可能影响对IOP的敏感性。需要进一步的工作来确定观察到的卷曲模式的生物力学影响。相对较大的变化，卷曲形态的利姆布斯，特别是在生命的早期，这表明，卷曲在这个地区可能发挥了作用，在眼睛的发展，虽然这是不清楚的确切性质。
Collagen is the main load-bearing component of the eye, and collagen crimp is a critical determinant of tissue mechanical behavior. We test the hypothesis that collagen crimp morphology varies over the human cornea and sclera and with age. We analyzed 42 axial whole-globe sections from 20 normal eyes of 20 human donors, ranging in age from 0.08 (1 month) to 97 years. The sections were imaged using polarized light microscopy to obtain μm-scale fiber bundle/lamellae orientation from two corneal and six scleral regions. Crimp morphology was quantified through waviness, tortuosity, and amplitude. Whole-globe median waviness, tortuosity, and amplitude were 0.127 radians, 1.002, and 0.273 μm, respectively. These parameters, however, were not uniform over the globe, instead exhibiting distinct, consistent patterns. All crimp parameters decreased significantly with age, with significantly different age-related decreases between regions. The crimp morphology of the limbus changed the most drastically with age, such that it had the largest crimp in neonates, and among the smallest in the elderly. Age-related decreases in crimp parameters are likely one of the mechanisms underlying age-related stiffening of the sclera and cornea, potentially influencing sensitivity to IOP. Further work is needed to determine the biomechanical implications of the crimp patterns observed. The comparatively large changes in the crimp morphology of the limbus, especially in the early years of life, suggest that crimp in this region may play a role in eye development, although the exact nature of this is unclear.
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影响因子：
3.5
作者：
Fazio, Massimo A.;Grytz, Rafael;Morris, Jeffrey S.;Bruno, Luigi;Gardiner, Stuart K.;Girkin, Christopher A.;Downs, J. Crawford
通讯作者：
Downs, J. Crawford
影响因子：
2
作者：
Grytz R;El Hamdaoui M
通讯作者：
El Hamdaoui M
DOI：
10.1115/1.1427698
发表时间：
2002-02-01
影响因子：
1.7
作者：
Hansen, KA;Weiss, JA;Barton, JK
通讯作者：
Barton, JK
DOI：
10.1098/rspb.1972.0019
发表时间：
1972-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DIAMANT, J;ARRIDGE, RGC;KELLER, A
通讯作者：
KELLER, A
影响因子：
3.4
作者：
Elsheikh, Ahmed;Geraghty, Brendan;Rama, Paolo
通讯作者：
Rama, Paolo