Biofilm-forming Staphylococcus epidermidis expressing vancomycin resistance early after adhesion to a metal surface.
Biofilm-forming Staphylococcus epidermidis expressing vancomycin resistance early after adhesion to a metal surface.
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DOI：
10.1155/2015/943056
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发表时间：
2015
影响因子：
--
通讯作者：
Osaki M
中科院分区：
文献类型：
--
作者：
Sakimura T;Kajiyama S;Adachi S;Chiba K;Yonekura A;Tomita M;Koseki H;Miyamoto T;Tsurumoto T;Osaki M
我们研究了表皮葡萄球菌粘附到金属表面后的生物膜形成和万古霉素 (VCM) 抗性表达的时间。使用 VCM MIC 为 1μg/mL 的生物膜形成表皮葡萄球菌。将细菌粘附在不锈钢垫圈上，并用 VCM 在不同时间和浓度下进行处理。粘附后0、2、4和8小时施用VCM。根据VCM施用前后的生物膜覆盖率(BCR)评估生物膜形成的量，使用荧光染色法目视观察生物膜中的细菌活力，并测量生物膜中的活细菌计数。与未经VCM处理的细菌相比，显着降低BCR所需的VCM浓度为4μg/mL，即使在0小时组中也是如此。在 4 小时和 8 小时组中，即使浓度为 1,024μg/mL，VCM 也无法抑制生物膜的生长。在 8 小时组中，即使在高 VCM 浓度（1,024μg/mL）下，生物膜中仍保留有 104 CFU 的活细菌。有人认为，形成生物膜的表皮葡萄球菌在粘附到金属表面后早期就表现出对 VCM 的抗性。随着生物膜的形成，粘附后随着时间的推移，阻力增加，粘附后4-8小时表现出较强的阻力。
We investigated biofilm formation and time of vancomycin (VCM) resistance expression after adhesion to a metal surface in Staphylococcus epidermidis. Biofilm-forming Staphylococcus epidermidis with a VCM MIC of 1 μg/mL was used. The bacteria were made to adhere to a stainless steel washer and treated with VCM at different times and concentrations. VCM was administered 0, 2, 4, and 8 hours after adhesion. The amount of biofilm formed was evaluated based on the biofilm coverage rates (BCRs) before and after VCM administration, bacterial viability in biofilm was visually observed using the fluorescence staining method, and the viable bacterial count in biofilm was measured. The VCM concentration required to decrease BCR significantly compared with that of VCM-untreated bacteria was 4 μg/mL, even in the 0 hr group. In the 4 and 8 hr groups, VCM could not inhibit biofilm growth even at 1,024 μg/mL. In the 8 hr group, viable bacteria remained in biofilm at a count of 104 CFU even at a high VCM concentration (1,024 μg/mL). It was suggested that biofilm-forming Staphylococcus epidermidis expresses resistance to VCM early after adhesion to a metal surface. Resistance increased over time after adhesion as the biofilm formed, and strong resistance was expressed 4–8 hours after adhesion.
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